Mosavi, Seyed Jamalaldin

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D ThesisTitle:Formulation and physicochemical characterization of solid lipidnanoparticles of Dimethyl FumarateBy:Seyed Jamaleddin MousaviSupervisors:Dr. Mehdi AnsariDr. Salehe SabouriDr. Maryam KazemipourApril2018 Thesis\t,.:ut-st;G o--l);:a-2$;"jtlc ,: T ,SGJ:L ;i ;: o5 *l ,;*-lry- J-,L* .fy (MS ) u*,)jr(-l JJt g;L* :+"-t,l-.6- t:-g ,r;-ae i- 1t-._t-1;K :rr-, ; ;1 ;.c! iG) ;Sf ! s ,:f l^- u".-"o .gG J} .-.:l;L; "[- 6, g31\: :3;u" o-u;S ry-o ;U^- .;]t5 C--eii oi .S_,L* -e\ ,-Slsf .gLaoL;: ;-, Jk-l ,.r^"(*l :;r -uiL ,r-rl-S J; * se:\3o,Jlrl, ;-,1> ;l J,*{ J:* f} 'c*1 (DMFt- ;\ u:;-! :-,1> ;1 ;r-*^a .>y-€'rlrL* Fl -,, ;*; ; ))j jel.y -l U)+ ;--\-s gl;i;l:,SL^ f^*-" )l 6i.>;l: u-^ao f---:- -r) ",j .grK-uG : :y.,r/ )) ,S),--.s-*s "f:l:o -u\y€ a5;-l (SLN)-r"t+64;l;i ytJ r, J;1, S#,,\,{Jz cSL^ ,-sy-lu-:.-u-< ;lr-- lJ DMF .gLa c--:y-t=-!,*r-.:3*:t o:Li:-l oi;l uE-r+ J-\y S\t U -u;;"jJ 3;l: l.rnu* l-u,l ;",c;;l ;::.1t5 -;-.-.-r.; q{; r1r, 'iI; .rL^ -Lr-,> \ isr-l:,; l< .Jrll-rl : U! oif -,L:! ! ',;a-[];-..o -*-,-.g[a *]k5G; -y- i)J.ri5 q ! 3 r; o>Li.il -t"t? -r=..l Jlp rWitepsol_H35 ;l ry*U-- ; _- - -3-ti ,o\st:f oKi, ! .r-t- .-r..;-t- ,l r,-L J -l-<; JlriJjU Spane 80 .Tweens 20 .Tri .., ' ,.-t-.--rl,*.ll ;"it, o1/V.gl ";i ";l-til ! ox-U_r"l J -*ra;,::!'GJ-".-U--; i:-) :- --i:*-|s"}at"^Jri;*:,l"^a,.;,'-ys.y|Y\Y/1;.1r.9l,,o-t"l!:-){./-a.;--a..>:yt.Y".l^ir.\.jl}:ls,-|s,.c),)s\>J-lL:-..,c3VJ's4*'-o-.j-j-=II u.-)G 4--.U;,S-t,fi JrS S:S >lri,,,y o-r^; >l;i,-t-"i.-*, ! d+)'\. jl -ur /\q 4t orL J-J- 7;1,---'juJs -W; /\1 a, t:r-ls,.p;l o-u:l;i gy;l: .,1i:" +rl-ir Yi' ji,j rr .-r::7.a.-t-:rS;.-ir .:;:,<"-, .S-,1{1" -tt-J ,l ..:;1.: .g;L>lji .tr,q e9.. )l jLX 45 ), o{---r /YY a, J}^" ,;6)^A ) -J"p;-'t'.-ta; "L L rl -t'i 4< -t; o:1:;l; i)\ J L*,Jl-n- g;L: Y ;: Ji"-l-a:/,.)uoi-\ C..^a 6 j). J VJ-- u.-r--A. iL-.c--z;.-gl;l.: o-u; o"+ ;:..U-r, t .J;; -,l -., or"i +) 4. Gtr q "+:, ! 4$ ^--:.)y- ,s-)Jinvivo o.? )invitro ;s 1;ls;.-! ! +--Li,;:;i.-l;1 d rl. u: a*y lJ y:-u\ro -* .-u"t- -.+,.J ;'r! -:Li ,elrL.i J* J) ._--;;,J-1, L*:it :J{5 iL.JS-t^-^; X- *,e-- - -*-lK;t +-.alj;Abstract:Introduction: Multiple Sclerosis is an autoimmnne disease in which T-cells attack asain.:neuron's Myelin and the efficiency of nervous system is decreased over time. One of the .',r:.tnedicines for MS is Dirnethyl Fumarate (DMF) that treats relapsing-remitting MS. DMF hasseveral adverse effects like abdominal pain, diarrhea, nausea and flushing. Also, D\fFpassing through Blood-Brain Barrier is difficult and has low permanency in nervous svstenrOne of the new drug-delivery systems is solid lipid nanoparticles that can compensate th;adverse effects and the passing problems of DMF.Methods: In this study, first, the hrr* of DMF was determined for using in the next stage sThen, several forrnulations were prepared with various percentage of components. Hig:-shear homogenization and ultrasound method were used to prepare the formulations. In t}. .stage, the solid lipid was Witepsol-H35 and the surfactants were Tween'e 80, Tween& 20 a:.:Span@ 80. Then, the sizes of nanopafticles were anal5,zed and a formulation rvith 56.1 r::average size was selected for the next steps. Also, the formation of nanopafiicles ..''confirmed by optical microscope photography. At last, physicocherrical propefiies of . .best formulation rvas studied.Results: The valr.re of tr-u* was212.9 nm. Encapsulation efficacy of loaded DMF rvas 5-i -formulation diffusion waslo/o after 60 min while diffusion for solute fbrm w-as l9oti.lt n,:: lthat forrnulation is controlled-release. The amount of drug that was released after l-,- : -was about 160/ob.ut for solute fonn, it was22Yo.lt means that formulation is a sLl::-::-:--release. Furthermore, the physical stability of the formulation was investlgate; :formulation was stored in refrigerator, rooln and oven temperature rvithout anr ;hr::=,color and consistency aft-er 4 month.Conclusion: Based on this project's results, the selected formr.rlation sl',.-i,.. :, : :characteristics. Therefore, it is recommended to investigate the in t'i/t'o and li; '. :'. ;r : -the SLN formulation in comparison to DMF powder.ilIIVKey Words: MLrltiple Sclerosis. Dimethyl Fumarate, Solid Lipid \anri::: - .physicochern ical characlerization.irt Jt dL;o =lil*e etc.ti 9 ..Ji1l, nt& clSiilogjt r.rls c.tiiibtPl o1L*i { Al gs9.19.911r911s.9#ilo g3lr. Of.tJl Jh?a+*.gtiT &li drQQ:glr. sri' *t ti,f 11 t 6,b* 64,u; ;t d.i 1 t? i rr + u/ t4'{uafl.tfLJcrJ4.. i5) *1r,:-JL 5; -Y:ti,t jsr tl9to p;ru *LSX.r,t"rt,-1..l . ^1r ., ;l^ ^" -(r-l6)'*.)y,6-r.,-),G;f.: -Y, P d.V.t.i+d i5l -f$7(.A*-....... (:-r-' t-r) 0.,* q t uf M-yff;) 2t*1v/Y/.t 1r* :lrJ, -r.io, so*a dt- / , -J

فرمولاسیون و بررسی خصوصیات فیزیکوشیمیایی نانو ذرات لیپیدی جامد حاوی دی متیل فومارات

http://eprints.kmu.ac.ir/27303/1/6543.pdf

فرمولاسیون و بررسی خصوصیات فیزیکوشیمیایی نانو ذرات لیپیدی جامد حاوی دی متیل فومارات

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