Method Development In Assessing Recombinant Human Growth Hormone Expression From Pichia Pastoris [QH443. C434 2007 f rb].

Abstract

Hormon pertumbuhan manusia rekombinan (rhGH) telah dihasilkan daripada strain Pichia pastoris GS115 2(5) yang telah mengalami pengubahsuaian genetik dalam proses pengkulturan dua-peringkat. Recombinant human growth hormone (rhGH) was expressed in genetically modified Pichia pastoris strain GS115 2(5) in a two-stage cultivation process. However, the preliminary cultivation process and purification strategies of rhGH from strain GS115 2(5) were still an open issue