Rigi, Malek Hossein

Simorgh Research Repository

,lLlfi/',il,ttd:,-Ih.6-, o. r( .i.'.1.1(ss-,,i o-sli.i:Sf:. c.:-:iJ dJf (s.lJlli,iJl,S C.[,s6 o".t U!!,l-&csLn!J-, .2r blagTy-^a nj JSls. ,-,n+fi o 51,31* FT *.ll* ,s-6ll ,f)icrlJL":# 3l o.l,}, l;- (ESBLs) ;,t^t1 G-,J 6La;LtX'jE o$,S +tS :iSl*sFl;l"rat; ,'-,JJ*I c;La;ll ',.rt-"*, q o.r,K c-*..lJ.,,Ql ,,1,,rs Slo:L,,.JiCJt-i ff J(ri;iS.r - g;.!lJ.o d.,:-o J.3So :L&1.2 .ll.i"'l\flt-\flv:oJ*asi Jt-l(Jkerman Universify of Medical SciencesFaculty of MedicineIn Partial Fulfilhnent of the Requirernents for the Degree MscTitle:Determination of antibiotic resistance pattern and morecurardetection of blagrx-M gene in extended-spectrum beta-ractamases(ESBLs) producing Enterobactel spp. isorated from patientsreferred to educational hospitals of ZahedanBy:Malek Hossein RigiSupervisors:1-Dr Mohammad Moradi2-Dr Shahram ShahrakiYear:1396-1397{f i blagly-Nl oj d#y.dL-l:i t .5g*.*i c-9U; 6#l e).f Z.} ,-r-l1l -iro :a-,ia: a.r-5 a-*lr,.rlrL*t 1l o r ; h_> (ESBLs) ,--o *Llla *9 .5Lo;LhlYLi ol5rJ9; -fl.g.*' :-.;y ;hall d)ri.sl"; - . -a-;l-r-ol1 d)yi.5Lo.rl.-,110*.t p 6i-*.rl,,l--* ^<Ly..t' *!t+ o-+,- *i9):bc.rpl.r 9 )1r-r.#111.*-ji ---tD g c.-sr?,'l-:o-il ,r1ll.l ;g*.1 ci"r-*,-L' .5Lo,--'.:' .$ LL;!. .r-h ?g* "L(---..dl-.: l.pK-i_t,i.5to:rlSb*,1 a ^9a.51-ur|-Jlrf-) d-? qil-r.tjf+-:,-(---.: ,s"St + Lr -,._--&ar-.r:i a-, a-1 (c*9U. QA-"y. - r ,,L.-') qf-t-- dia5 r-+lr .rL" J9;-l .r; pL;liCLS-#; (<+r_) ,-t,St )l o)1;: ...1 I , ESBLs 1f S $ri sl->';jl ;y't_;n- 9 ,r.r-jl., ,,. ,p--S':i-;tsl; ,_y'g; A-.blag1y-Nt 6 )*9 P )ESBLs .r+lf .sl_o a-19;l ,c--Q ,; .c.-srS ;1.# s,t r,-.!,if )p ,*ty rre- (PCR) ;ls.L- 61,.;-Y. ,,j11ob-;;9,r*sL.-.rtt-rU c,G-:i; ,r;3 ,rlrt1,,jt' ) o-r-oic--i,;J! .;:^" Y" ;l : e.r-lJ9 prr,-jlk .,. o-ltrlr,,..-Jb+ o+/.v:lr ,f\sr.l dsxl5l ra jl .-ri.:y F4srJ (lra) "-. --..r-i.:y c*rr ESBLs :;9 $:;l (/Vt/f) a-J9;l A. :l-r-; .r:rl.: ,,1 ;', ,--"91- ;r-t\.iL" o-.'t.i,' '.CTX-M-I oes-S;s/.ff o-S sjsy bloqx-u ;j r->le L-o dst-| (lb?)\^,c*--ia ESBLs a-Jt;,-' i'.r;rf )llcTx-M-IY 'ef ,rlt scTx-M-III og_F il?,CTX-}/r-IIoef ,:'.'cSL6 c1,rf,/ll- o: crJ Yt-.. &*-.r-:i c-"91", a-l.la:,--",jL.t-' Fl> a-ltt" z cSF s.;-u- ESBLs !f .ri .sL" F--nti jl ,-.<- aJ rrl: :9-'9 ;Lol; F\sr.l.5Lo +J9;1.,: , ; .Lll a-..:u;tr ,r" to al9;-l ;-l 2r ta rot,pi "r+l o.ri-ll csta Oj ar)*{d )l blactx-xt o: : - -CTX-M . ESBLs , ,$y,ei tu16 ,F\syl:.5qlf cltolt.*.rG !J.>.>AbstractIntroduction: The aim of this study was to investigate of the antibiotic resistance pattern andmolecular detection of blacrx-yt gene in the extended-spectrum beta-lactamases (ESBLs)producing Enterobacter species isolated from referred patients to educational hospitals ofZahedan.Materials and methods: 200 clinical samples of inpatients from educational hospitals ofZahedan was transferred to the microbiological laboratory. Biochemical confirmatory tests wereconducted and antibiotic susceptibility of isolates were done by disc diffusion method based onclinical & laboratory standards institute (CLSD guidelines. The isolates with decreasedsusceptibility (intermediate or resistance) to three antibiotics of cefotaxime, ceftazidime, andceftriaxone were selected and ESBLs production were evaluated by combined disc method.Finally, the ESBLs producing isolates were investigated for blacrx-ugene by polymerase chainreaction (PCR) method.Results: A total of 200 clinical samples obtained from blood, urine, catheter, mucosal-tissuesecretions and vaginal discharge, 70 isolates were Enterobacter. Of the seventy Enterobacterisolates, 71.4yo, 64.3yo, and 68.6Yo were resistant to cefotaxime, ceftazidime, and ceftriaxone,respectively. Fifty isolates (71.4%) were positive for ESBLs. Out.of 50 ESBLs positive isolates,28 isolates (56%) have possessed the blactx-11 gene, that 44yo, 40 , 60/o, and 2o/o were in the CTX-M-I, CTX-M-II, CTX-M-III, and CTX-M-IV groups, respectively.Conclusion: The present study shows that high antibiotic resistance to broad-spectrumcephalosporins exists in Enterobacter isolates of Zahedan that one of its mechanisms is theproduction of ESBLs, and the blagyy-\t gene is one of the most important genes encoding theseerzymes in these isolates.Keywords: Enterob acter, Antibiotic resistance, ESBLs, CTX-M.t-" " "c--J-.,..,..,..,,.....orLj..,....,......,..C+u otof '3tuJ lrJc .lllroL(ii{s .,lA$ ir)'--'oK-i1, &i.r*;|, r#ti,il !JSJ-(F a*) r:,)l ,-*is{ ,927.-:;\s.-#-: CXi."t efln 6L9T JL$; e-U rtL.[, ccti: a.J=rgb 4--H;s btactx-M Oj JfSf U..rr*r 9 *$jX .=;T c-ogGo 6g--31 t#);." ;)trr i; iuf Ji; ,.f,.J)yT gSteh*ril.od a4 o*;f +-r7ly r.ll:t-** jl o"r.i l.r= (ESBts ) *+{ tt C.+g 6L$jtokfyb o*-if .A +r9j JJ1+fil J*;,,-rl-,l-el: oLo (p6Wl )t4-L.11/ l1 llY g_r qiia-,j;rl.:f. c^cL -,1r t'g -s--{,aat irstft; fl;r fu C.ArJ.-vI6clJ..l^rs,o Jifo rSGT ?t ":Jjl qgLal.; : L-l:,--cJl{-,/21*,f 2* flrei is,s AGT Vlq le qr , cL.i.al r rh-l iu\- -:1311- :L-ll :dtr{!^i Lb;.ra.ao.rJs 6tiT r-r1.7 (,_rLl:) dlJ3l: cJL^ t-2-c:,-:'{ "';'* r.S.9-tgr oalll c^r-l 0$t .l#;fc 6GT r-.rL> (,-re-lt-) gl;r-9lr 9Ln 3*-c:. :dffi ,.#: cst)sf ;rc frb ir t"t ,rt-i crX.+; c.$L; : o.*f tts+-'u,r:..........\.Y1..1 f. . . ,* r.......n-b;L-+ 4-)5 ! *y, Cr- *.e.r-)st a,^-? r,1,,: --oJlri ao*la"U Olr-t";l glsc *,*Iacily:offiq*e*gadddf*7

بررسی الگوی مقاومت آنتی بیوتیک و تشخیص مولکولی ژن blaCTX-M در سویه های انتروباکتر تولید کننده بتالاکتامازهای وسیع الطیف ( EESBLs  ) جدا شده از بیماران مراجعه کننده به بیمارستان های آموزشی زاهدان

http://eprints.kmu.ac.ir/27045/1/6460_20180408_0001.pdf

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