Foroghi Borj, Hamid

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au|{,,(P,0,,,,1* .rl,iil *.r:4-,rS,},i oaS*i.]l.l.,S,ii .,*ti,i JS.I {:,iJ .rill ,r-t'.,l.JtS 3t3. *U al*!J j.J .$i,*,35) "d efi crt, th ,-..r.;( -r.r cg$j t -.,\J-*S,1. ef-r$ .5*,r;nt+J" ;E -,;d ;.r PCR .l cgjJ-r-)*, ,.ri.lt 1+ (,llSGJn tfJ$ J"= : L "''il.+ ,l1j ;".ol-, ;iS.r.llj: 6.u*l J-o.-3o .,!Sl :tnal; .lti-,t.r:lftt l-,at j(.r-.,q-d 6-,11 -nS.r :;r3tI* .rti*,|\Ylo-11 : ,l*,--: Jt- ?Kerman Universify of Medical SciencesFaculty of MedicineIn Partial Fulfillment of the Requirements For the Degree (MSc) in MedicalParasitologyTitle:sunvey on frequency of roxoplasma gondiiin livestock meat (sheep, goatsand cattle) by using serology and pcR methods in euchan cityby:Hamid Foroughi BorjSupervisors:1- Dr. Mohammad AhmadinejadZ-Dr. Naser Zia-AliAdvisors:1- Dr.Iraj Sharifi2-Dr. Zahra BabaeiYear:2018'l-/o+5?9 tt olll;. c-! d,e.-l,jt,qa,,1;ll* .'Sqifl rutr cr.rtql4.r-jr-ytrJr, :,-irat9 AoJLgal.i.ra.c*,lory'I pB t. {+ .;f ot5s a;il,5, Otl Jlirl ,.5to oll d.,g^4d;l.,5.1.rr d ci)t-j il"0,.:i,.3 gV-l Je**Wygl c$ly,ftt, \ sV s i ,ui,-eS st 6sf t->!rfl .*y t* O*0, a-llt".rrJf t-)t*9,J, J(;l \--tij A#+ c4.l4j 1) 9 i*r.a p)- pl) .rLo c-it{,,) JftIDNA d4}*^ii', (MAT).)o\?. t t \YA,r,!i-,5 \rA J"tJ) pl.: fAF jl vl! I pfl,pt*, cx!.st" st- s Of crta dj9- tb.-Fflp)- crta .j,.i ..r"i .519'i 6.; \Ytt JL oL ;yrle! b \fld JL oL rl:r. ;l Obp OUXp ol5;hJ;l (9ll\:Y.< .5,:! ",d i* ..rc)g )f e)f; ))r MAT ;r, \ asf t-\lJ, qJ" .sr! ;1 ,.*=,s cap,sst yBl6j b Nested-PCR di9) ;l js:;IDNA Lta*r,:; .4e d.r", ,.l"i a:i.f,,t; 1.: c.:" a;4 ;19,.c +orlo:.,11PCR-RFLP ,-y'y,&\.#"U'ij,Je.i,i;qA 01 ,:o.l ..ri or[o,,rl ,c* Fp pb oflrllr, + &e1...$,5 rl2.;lsequencing *1.hr.D 9 GRA6 uj;llta2 u lay ;l (\YA/r. ) lYY,f ,gl.r:r*,el ;l (r2ilfP) IYV,F ,.: MAT .5jle2- .r&t, 6-."U; :b ciL,-tE I y.,r;;-99 r^t, I fl,ptr, dt" c.!ti 1r 81 gj U Nested-PCn ,nUJ e-tt .ri c,.:" ta ,15 jl (\t'lYA)(yA/y)z\Y e (YA/P)lYf ,(Y./Y) lYYf e r./\Y)lr. ,(rrl\Y) l\?,: t ftlYY) lrvtrt *; { '+" Fr'Y ,ri-9t V) pb \Y ,l ,'5lri!..: I ..ti .rl^ a;9at 6il)? GRA6 ;j L Nested-PCR Aglx 4p ..t"1 ,:-sI y-l;yj t" o|.;r,d Aaa ,MseI .r,i21 ,"{,i,1 , o.:ti;l 9 RFLP ls(rsi'11 ortril t? 4t ,Jj c,,l (9tl Y 9 ;I v,-ti' + 4.r,, t" $g* a a.a DNA .rlr gL;; .(rjr-, 9 d-lfu ;JU1 .a:2f O*.- &i9, t-)!'lJf,,>-l ,5595 t*,)(i#ya;b- d/-l i gV t ,1 ,xi,*99 i tssf t-)t9,Jy Gl# 6 rlr gLl a,Jtt ,.14..............1 a,U;:r5f aa,-;i&Urr +lr ru-, t?. e pV df 0:* ,-y.b :l ,ssf t*;U,y-fy sr* q V+l ,J.> .Ji.oK c.te e c*,VqRFLP-PCR ,B1 gene ,GRA6 gene rNested-PcR ,MAT qsbf t-\#, :ojl9 {lfaAbstract:Background and Objectives: Toxoplasmosis is a major zoonosis disease with globaldistribution that causes a lot of casualties and financial losses. One of the most important wayto transmit this Protozoan is to eat uncooked or raw meat. The purpose of this study was todetermine the seroprevalence of Toxoplasma gondii in sheep, goats and cattle by using themodified agglutination test (MAT), and parasite DNA detection in tissues of seropositivelivestock as well as determining the genotype of Toxoplasma gondii.Methods: Blood samples and diaphragm and heart tissues samples were collected from 456animals ( I 68 sheep, 128 goats, and 1 60 cattle) from slaughterhouse in Quchan City from August2016 to Apil 2017. Serum samples examined by MAT method to detect antiioxoplasmaantibodies. Antibody titers of > l:20 were considered as positive. PCR was performed toamplify the Toxoplasma gondii genes of diaphragm and heart tisuues of seropositive samplesfor amplification of single-copy polymorphic loci.Then, Nested-PCR performed to ampliS, thefregment of the 81 gene to detect parasite DNA performed. To determine the genotype of i'.gondii performed PCR-RFLP method of GRA6 gene. Also, sequencing analysis was performedto evaluate the Toxoplasma type strains.Results: Positive MAT results were found in 461168 Q7.4%) of Sheep, 301128 (23.4%) otGoats and 251160 (15.6%) of Cattle serum samples. Positive Nested-PCR of B1 gene wereobtained in diaphragm and heart tissues of sheep, goats and cattle were 22146 (47.8oA), \2146(26.1%),12130 (40%),713A Q3,3%),6125 (24%), and3125 (12%), respectively. Nested-PCRof GRA6 gene results were positive in 12 samples (7 sheep samples, 3 goats samples and 2cattle samples) in diaphragm and heart tissues that RFLP technique results with using MseIenzyme revealed Toxoplasma genotype I (12112). Sequencing and Phylogenetic analysisrevealed DNA of all samples were related to Toxoplasma type l.Conclusion: The results of this study indicated frequency of T. gondii in sheep, goats, and cattleis high in this region and preventive program to reduce the risk of T. gondii infection by raw orundercooked meat consumption of sheep, goats and cattle should be considered.Keywords: Toxoplasma gondii, MAT, Nested-PCR, B1 gene, GRA6 gene, RFLP-PCR:. l:" "'c--J'. ................ orl. ::r..,.,.,......f.og!_lLrl as-.1+"U Olr-Q;l glss @1yOtoy' ,=1131, ;& ;3'-otii.;ls,=lo.G J)---g;rlo .K:;t, ,-#i,.K.$l-, ."*rt.i.!51 c.;,, J-.irl t,-Li,rlt 69a-:rl: sjlgfJa\at rJitJT,rL""*' '-"u .rL-t, a-stir a-'b,,if2 f+ ( lS g y.. J;i-tif) o;u,i C-ls .gt.l Fls cirf Js 1.5.lugf h*ryr,rry e{jf \#)J." .1lr-ro d-*' Jl-f ;Gi, J*r" trl-;3tr c,Lr pf* a;tiA )r;- L. tl/ I f/A &,. td dr' )ss t ' d;"cL t, " Ol?t' OUrJd ;c PCR I 6,3grlf-p +-L- )rr, .............' I lr .-*......'.... .r^; , ..... *-rJb;L-t: """ 47)'L. 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بررسی توزیع توکسوپلاسما گوندی در گوشت دام های ذبح شده ( گوسفند، بز و گاو ) با روش سرولوژی و PCR در شهرستان قوچان

http://eprints.kmu.ac.ir/27084/1/6485_20180408_0001.pdf

بررسی توزیع توکسوپلاسما گوندی در گوشت دام های ذبح شده ( گوسفند، بز و گاو ) با روش سرولوژی و PCR در شهرستان قوچان

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