Ghasroldashti, Zahra

Simorgh Research Repository

,YoVb_$j:ie"nw- 6-l/,- '''1.'P.. l.,,tt(/r,aPot,,,,(,.?,-;4 o.rS,i,ll.l.rSi,+ ,r*,1'.,:, o-JlJ .l,irl ,r*,1'.,:.JtS Ct3- *lj "!!:;13',c\Y1I,;LTS,HIV q $.,- ;lrl ",.. ,.y; lji.6. C .$q^ .fl.llr, dr*:r,si,:JlJ,-3 t;a-, : L. ,.r:.r:Y-f L'9.1*-.s.;,:Sr o;l.; .r.,p .-L^^ -rlc r-r'. JiS.l ; l^"at, .lti-t\Y11-\Y1V : .J*,--: Jt-,kerman University of Medical SciencesFaculty of MedicineIn Partial Fulfillment of the Requirements for the DegreeMScTitle:Evaluation frequency of occult Hepatitis C among patients with HrVpositive, kerman,2016By:Zahra GhasrodashtiSupervisors:1- Dr. A.M.Arabzadeh2- Dr.H.R. MolaiYear:20r6-20t7orsrHuman immunodeficiency ) OL;I .;--l ,--; d9.r-9 (co-infection) At;* c;rro :e!l.ral 9 a*..r-tat..rlrt* r.: .:9;*".s{.rt^ -o,,i c-rr* * (HBV) B , (HCV) C ,r-sy-g +1.^ }lr ! (Virus"lr* .s+J +* ti (HAART) Jtr ,!* Lr.rt-99;) lj ;Lrr 4ritt C -*L" I HIV ;L;.,r c;9r;yL:- a". r;L a,Jtt .::r(- HCV,HIV ,t,p cip 0". )L- ;111'"* .Sy -N ua- c,\;l do. )Zc,a aS ,c)>.$L- HIV c;rrc + ).,t;-.-,1,,L,: .,, d;U C c,*L^ q )k- .:rl9,.i-L'';,(ALT) );.;li ry.i ;Yi .si^ ,o,-ri eb- .cori ;:ry- HIVa, ),{;- )1.*\ . - ;\ ,sF dr- :kJ:,ajear 1) HCV-RNA )e;> ,.); 6f 011s1 (ALK) ;L'U,.-r ;JtSJi ,(AST) J;;,l; e;.,"1 ;L'r-U* tr 9 HIV a, )k* ,,1,,L* .sL-\ .i9- ,: HCV-RNA ,t;> 9 Nested-PCR "n2 a, PB\ICs.u.F rn- RT-PCR Gs: * PBMCs 9 L-)L ," :io.5to d9- r, HCV e=;,-.,L)^^ .;9;s ,gl)t .rlrL* jl F YY 4t .:b ;L;; HIV a, yj- Jl)t , Lr,)t )l J-l- e.b :Ll a:il"-," :," C .-:rF -,L* (41/\)\r 9 {r{c C .+1.^ ,t.i (Zf '/1)1 .r) p YY 69*. ;l ,o:9, HiV e Cc;,-^-!!-:;.^rq:xY! -+^C:J'I.^9Ar{cC.+t ^+V:-.,I,,LorLJf e$.ih.--}i .-rjo:b ra:>-C c-9[. .rlrk* I a:'itr Q ;gtr ! ...,1,,L*.,: (ALT"AST.[K).s+J .Jhc"-ii eL .x .5;bL, t--c+L^ jl ;Yt c*i C c+'t ^ a, )k- ..,1,,L* .,, .jj$ .jk --if eU- af+ .P: 0.64) )rl.r; )9.9 ;-j.jl ," '," Cc+q" + )h- ..,1,,L.: ,,: (/.f21\)1a 9 a:rv C.+Lo ..rl,,L* .,) (lfflf)3a t*2j .:2 a:1C,iyjti gHCV o"9t-9)UHIVi HCV;L;^o -i1s.oa'.!:,-;1110*1:.rir9r tlrrft6r;*,:1't:)*g rs,gl.g :\ I =*fi ty o*,-5rlrL:--" tL;,| .5.,Li 61h; r-Ll ,1 .r;$ a--to. rf---r.-o t? L*)t , -7.-.5111r;l,L"rr ! ^--t,L p ,st$.rk--i1 et- HIV/HCV,t.;'l c;9* I{,,1,,L*,,r .(P:0.47) :.-.(pr<0.01) 19, ,lr G..'/. \ et- ,r 9 c-ilr .+.lil .t-k* or Fllt'-{. ,:,;*r al^ ,s,;.r L.rb;L;lr'- r." HIV ;l,,1.,,. rr a;4i C c+'F )l.y dV 6'1 :6yS a->,-.rf sf i+ HIV t 4r * s* tSr )1 ;|i*;L;.:;: i c$.sb..-"-i g F,-b|y C;; --PBMCs ,(Occult HCV)*;; C.+t" .(Coinfection) .ltF r;*t z,S$ JL..5AbstractBackground of objective: The co-infbction of the Human Intmunodeflciency Virus and thelactors of HCV and HBV caLrscs the development of hepatotoxicity. In patiants witlr HIV andoccult hepatitis C tltc active retro-virus treatment (llAART) colles along with hepatotoxicityand leads to negative inrpairrrerrts in patients sLrfI'ering fiom HIV/HCV. The followingresearch identifies cases suff'ering fi'om occult hepatitis C in patients r.vith HIV. hundredpatierrts with hurnan immuttodeficiency virr-rs (FIIV) were enrolled in this cross-sectionalstudy.Methods: l'he level of ALT. AST, ALK e ltz1,11s5 have been rneasured and the presence ofHCV-RNA in PBMCs rvith the rrethod of Nested-PCR arrd the detection of I{CV-RNA in theplasma samples of HIV positive patients and also the detenrination of HCV genotypes inpositive samples of PBMCs with the method rvas conducted using RT-PCR .R"^rufts, fu o1 lll pafilnk, // w/ink (z%l rtele su#nllg f'ont t/te co-nfrctbnIilV/HCV. The HCV-RNAwas found in PBMCs specimens irr 9 patients (a0.9%) outof thetotal 22 patients. also l3 patients (59.1%) outof 22 cases u'itl.t detectable HCV-RNA plasr.na.HCV genotyping anatysis showed that 4 patients (44/%) were infected r,vith HCV subtype3a,1 (1 1.1%) was infected rvith HCV subtype la and 2 cases (22.2%) u'as infected with FICVsr-rbtype 1b. This study revealed the incidence of occult hepatitis C (40.9%) in HIV-infectedpatients.ViraI load and plasrna genotpe cotnpared Lt patients with HIV/HCV co-infection.There is no significant relationship between the type of genotype and viral load based onstatisticsl results(p:0.47). This str.rdy has shor'vn the incidence of occult HCV in HIV positivepatients. ln patients with HIV and co-infectiorr with hepatitis C. the level of liver enzyrnesrvas significantly higher in 1o/o than in patients with HIV alone(pr<0.01).Conciusion: By doing firrther research in order to discover and elirninate the factors ofdanger and darrage of the liver during tlre treatmet. it is possible to prevent the possible derthrelated to HIV.Keyword: Co-infection, Occult HCV, PBMCs';tri f.-"- 'jl: , l*"U *t.!, ;l gl"i+ *.*J-aJ;y,:-4*! ;tt x15" e$*tzrMr.de Q,wf*11il1ilcir-;*:f.r,,,,';*r."H{v*:y;-r.,;,:Lo*;+ua6C;*fLs;,r,-* J#1-* --,:-3*.,.1li;l 3-:,r.i:j..l* ;:'d. :s;id: ..:-lex'trra :bd;*ry;s i *-"3;r * s.";; .* t ..{t,#f.:.:.4i-.""., "; -, -.." -* .*a..a::='.::.::,r lir::: i I ,. ....'"'"'.,..,.".s,1-:::. 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Evaluation frequency of occult Hepatitis C among patients with HIV positive, Kerman, 2016

http://eprints.kmu.ac.ir/27070/1/6476_20180408_0001.pdf

Evaluation frequency of occult Hepatitis C among patients with HIV positive, Kerman, 2016

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