Adlard, R.

Arango, C.

Couper, P.

Davie, P.

Hall, K.

Hooper, J.

Johnson, J.

Raven, R.

Worthington Wilmer, J.

University of Queensland eSpace

In 2012, the Molecular Identities 
Laboratory (MIL) at the Queensland 
Museum (QM) will celebrate its 10th 
birthday. Since it was established in 
2002, the lab has hosted research 
across a wide range of animal taxa, 
always with the overarching aim of 
using DNA data to assist the 
identification of new and existing 
species.  The primary work of the 
MIL is species-level discrimination, 
however, sequence data are used 
further to explore the evolutionary 
inter-relationships among diverse 
taxonomic groups.  
Accordingly, we use standard 
fragments for identification, such as 
the standard barcoding fragment of 
CO1 mtDNA.  Importantly, the QM is a 
repository for voucher specimens 
which are associated with the 
genetic data and we sequence 
additional loci, such as cytochrome b 
and 16S mtDNA, nuclear introns and 
18S and 28S rDNA, which are 
informative on a taxon-specific basis.  
By ensuring we have vouchers as 
well as an array of genetic fragments, 
our work is taxonomically relevant, 
verifiable as well as maximally 
informative. 
Crabs of the genus Austrothelaphusa, an Australian fresh water 
endemic group and the combined 16S and CO1 phylogeny showing 6 
possible new species. 
0.04
SSUfull_Kudoa_ex_brain_Lcarponotatu s
SSUfull_Kudoa_ex_brain_L_erhenberghii_RDA_4764
AY302724_Kudoa_shiomitsu i
AY152750_Kudoa_ovivora
SSUfull_Kudoa_ex_brain_L_fulviflamma_RDA4716
JF797621_Kudoa_pagrusi
FJ792714_Kudoa_kenti
AY152748_Kudoa_amamiensis
AY382607_Kudoa_thyrsite s
AF034640_Kudoa_paniformis
AB263074_Kudoa_megacapsul a
AY382606_Kudoa_lateolabracis
SSUfull_Kudoa_ex_brain_L_Lemniscatu s
DQ519390_Kudoa_ciliata e
AY302722_Kudoa_hypoepicardialis
FJ792716_Kudoa_paraquadricornis
AM183300_Kudoa_trifolia
AY623795_Kudoa_rosenbusch i
AY078428_Kudoa_quadricornis
FJ792708_Kudoa_gunterae
AY152747_Kudoa_thyrsite s
AB553294_Kudoa_iwatai
AB553300_Kudoa_thunni
SSUfull_Kudoa_ex_brain_Liza_vaigiensis
DQ439814_Kudoa_monodactyli
AY302737_Kudoa_scomberomor i
DQ519387_Kudoa_chaetodoni
AY152749_Kudoa_minithyrsite s
DQ519388_Kudoa_lethrini
AY641571_Kudoa_iwatai
SSUfull_Kudoa_ex_brain_Lehrenbergi
AB553299_Kudoa_trachuri
AF034639_Kudoa_miniauriculata
AY302738_Kudoa_thalassomi
GU124635_Kudoa_shiomitsu i
AB573715_Kudoa_prunus i
AY078429_Kudoa_permulticapsul a
AY172511_Kudoa_neurophila
AY302739_Kudoa_grammatorcyn i
SSUfull_Kudoa_ex_brain_Caesio_cuning
AY302741_Kudoa_yasunaga i
AF378347_Kudoa_crumena
AM490334_Kudoa_unicapsula
EU340234_Kudoa_amamiensis
Spores of the genus Kudoa, myxosporean parasites of marine fish, 
isolated from brain tissue and phylogeny based on complete small 
subunit  rDNA showing site of infection as the best correlation with 
genotype. 
S. chloroculus
Squalus Recorder Seamount 1
S. montalbani
S. grahami
S. edmundsi
S. hemipinnus
S. japonicus
S. nasutus
S. crassispinus
S. albifrons
S. megalops
S. brevirostris
S. acanthias
100
93
60
51
66
97
77
66
98
86
79
94
89
53
94
100
97
100
71
94
S. montalbani
Squalus Recorder Seamount 3
Squalus Recorder Seamount 4 
Squalus Recorder Seamount 2 
Squalus Britannia Seamount 1 
Squalus Britannia Seamount 2 
Squalus Britannia Seamount 3 
Squalus Britannia Seamount 4 
}
}
}
}
}
}
}
}
}
}
}
Species of the genus Squalus, a large group of dogfish sharks and 
the CO1 phylogeny identifying to species individuals caught on 
seamounts off the Queensland coast. 
0.1 substitutions / site
Werrikimbe NP (Boorgana) no voucher
Bruxner Park (Coffs Harbour) AMR160420
Sherwood Nat. Res. (Glenreagh) AMR158973
Sherwood Nat. Res. (Glenreagh) QMJ83591
Bruxner Park (Coffs Harbour) AMR158945
Bruxner Park(Coffs Harbour) QMJ83592
Bruxner Park (Coffs Harbour) AMR164146
1.00
Comboyne Plateau (Boorgana) AMR158991
O’Sullivans Gap (Buladelah) AMR163000
O’Sullivans Gap (Buladelah) AMR163001
0.99
Blue Hole (Armidale) QMJ83588
Blue Hole (Armidale) QMJ835891.00
0.77
1.00
Blady Grass Ck (Glen Innes) QMJ72343
Grange SF (Dorrigo) no voucher
Chaelundi AMR1419645
1.00
Ebor Falls (New England) AMR163003
Ebor Falls (New England) QMJ83593
Ebor Falls (New England) AMR163005
1.00
0.94
0.99
Gibralter Range (New England) AMR164153
Spirabo FW (New England) no voucher
Gibralter Range (New England) QMJ83586
Gibralter Range (New England) AMR164157
0.99
Queen Mary Falls QMJ80794
Queen Mary Falls QMJ80795
Queen Mary Falls QMJ80796
Girraween AMR157130
Girraween QMJ51633
0.98
1.00
1.00
0.58
Wyans Ck (Richmond Range) QMJ83584
Wyans Ck (Richmond Range) QMJ83585
Wyans Ck (Richmond Range) QMJ83583
Wyans Ck (Richmond Range) AMR164166
1.00
0.81
Brindle Ck (Border Ranges NP)  AMR158987
Mt. Warning QMJ83594
Main Range NP1 no voucher
Main Range NP2 no voucher
Mt. Mitchell (Main Range NP) QMJ80792
Mt. Cordeaux (Main Range NP) QMJ80793
Cambridge Plateau AMR158999
Lamington NP QMJ51640
Mt. Tamborine QMJ510950.84
0.72
1.00
1.00
S.salebrosus Blackdown Tableland QMJ51091
S.salebrosus Bulburin SF QMJ510900.99
S.cornutus   Black Mt. corridor AF109502
S.cornutus Black Mt. corridor AF109487
S.cornutus Big Tableland no voucher1.00
1.00
Branch Length
S. swaini
(Group 4)
S. kateae
sp. nov.
(Group 3)
S. moritzi
sp. nov.
(Group 1)
S. wyberba
(Group 2)
Node A
Node B
Node A
Node B
  
Species of the genus Saltuarius, members of a larger group of endemic 
Australian leaf-tailed geckos and the cytochrome b phylogeny 
identifying 4 new species. 
Marine sponges (Phylum Porifera) from the Indo-West Pacific and 
phylogeny based on CO1 showing inter-relationships of species of 
Class Astrophorida. 
Antarctic Peninsula, Scotia Arc
Eastern Weddell Sea
Antarctic Peninsula
Antarctic Peninsula
Antarctic Peninsula, Ross Sea
East Antarctic
Falkland/Burdwood Bank
Bouvet
South Sandwich, Bouvet, East Antarctic
Antarctic Peninsula, East Antarctic
Australian and Antarctic species of the Class Pycnogonida (sea 
spiders) and CO1 phylogeny of the genus Colossendeis defining 
species boundaries within Antarctic waters. 
MPT based on COI and 16S, Jacknife values(P=36)
'RR46Selenocos_
'RR45Selenocos_
'RR44Selenocos_
RR43Seleno
'RR42Melody_
'RR41Scras_'
'RR40Snumplu_
RR39Selenothol
'RR38Pcras_
RR21Selenoth
RR35Coremio
RR34Coremio
RR33Coremio
'RR07Pcras_
RR06Pcras
RR64Alongi
RR42Melody
RR32Coremio
RR28Selenoty
'RR25Pcras_
'RR23Selenoth_
RR22Selenoth
RR16Pcrass
RR11strenua
RR10Pcras
RR09Pcras
'RR08Pcras_
'RR06Pcras_
RR03Pcras
'RR02Pcras_
RR01Pcras
 Aphonopelma 
71
6
25
23
2
72
61
56
34
100
72
86
51
5
9
96
71
Spiders of the Infraorder Mygalomorphae, tarantulas, trap-door and 
funnel-web spiders and the combined CO1 and 16S phylogeny showing 
genetic affinities among Queensland species. 


Molecular Identities Laboratory, Queensland Museum, and its role in DNA barcoding

https://espace.library.uq.edu.au/view/UQ:98523df/UQ98523df_OA.pdf

Molecular Identities Laboratory, Queensland Museum, and its role in DNA barcoding

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