Several protocols of non-invasive transcranial magnetic stimulation have been developed in the past decades. Single-and paired-pulse transcranial magnetic stimulation are painless, and noninvasive tools to evaluate cortical and corticospinal excitability in cerebral cortex compared with transcranial electric stimulation. Motor evoked potential induced by paired-pulse transcranial magnetic stimulation can particularly assess changes of the cortical excitability after motor learning, such as motor skill and motor practice in sports and functional recovery in rehabilitation. However, the effect of electric current in transcranial magnetic stimulation on pyramidal neuron and interneuron in gray and white matters is not actually understood well yet in the field of sports and rehabilitation sciences. Here, we show the important basic knowledge of neurophysiology and transcranial magnetic stimulation and introduce some studies of cortical plasticity and motor learning by using transcranial magnetic stimulation