Figure S1. Unilateral intrastriatal injection of α-syn PFFs, but not RSA or PBS, induces bilateral cortical and unilateral SNc Lewy-body like inclusions of phosphorylated α-syn (pSyn). (a) pSyn pathology is observed bilaterally in cortical areas after unilateral injection of α-syn PFFs, namely in layers 2/3 and orbital and agranular insular cortices. (b) Injection of PBS or (c) RSA did not induce pSyn accumulation. (d) pSyn accumulation in the ipsilateral substantia nigra pars compacta (SNc) at 2 months postinjection, with no evidence of pSyn inclusions in the contralateral SNc. Scale bars (A–D) = 50 μm. Abbreviations: α-syn = alpha-synuclein; PFFs = pre-formed alpha-synuclein fibrils; PBS = phosphate-buffered saline; RSA = rat serum albumin; pSyn = α-syn phosphorylated at serine 129. (TIF 117729 kb
