Additional file 2: of Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions
Figure S2. Characterization of the bidirectional gene pair NFYA-OARD1. (A) The figure shows relative expression of NFYA-OARD1 in Jurkat cells as measured by quantitative RT-PCR analysis. The expression levels are normalized to GAPDH (B) Fluorescence images of the NFYA-OARD1 intergenic region when cloned into the pDR vector. The orientation of each clone with respect to NFYA is depicted below each set of images. Scale bar denotes 400 μm. (C) Flow cytometry plots of cells transfected with NFYA-OARD1 cloned into pDR vector in which NFYA is in sense orientation to mCherry. As a control NFYA cloned into eGFP-N1 vector was used wherein NFYA drives the expression of GFP. The axes denoting mCherry and eGFP are depicted adjacent to the plots. (PDF 1434 kb
