University of Zagreb. Faculty of Science. Department of Biology.
Abstract
Barkodiranje povezuje taksonomiju, molekularnu filogenetiku i populacijsku genetiku. Dokazana je mogućnost kreiranja baze podataka za životinje na temelju COI sekvence. Za druga carstva potrebno je provesti opsežnije analize u svrhu pronalaska najpogodnijeg barkoda. Daljnji razvoj i napredak ovog projekta omogućit će jasniji uvid u bioraznolikost, a analize su puno objektivnije nego kod klasičnih morfoloških analiza. Zanimljivo je i pomisliti na postojanje online enciklopedije života na Zemlji. Iako je projekt zapravo relativno nov, svakodnevno se ulaže trud da se premoste eventualne prepreke u procesima izgradnje ovakvog sustava. Metode u molekularnoj biologiji postaju jeftinije i mogu se uvesti u postupak barkodiranja. Razvijaju se mnoge nove metode poput pirosekvenciranja koje omogućava brzu analizu pomiješanih uzoraka. Već postoje i razvijene tehnike nedestruktivne ekstrakcije DNA iz nedavno prikupljenih uzoraka, što pospješuje njihovu konzervaciju. Sve prepreke moraju biti jasno definirane i premošćene da bi se BOLD baza podataka mogla u potpunosti uspješno razviti. Ukoliko neka organizacija poput Global Biodiversity Information Facility ili All Species Foundation preuzme ovaj projekt, to bi bio veliki korak prema dugovječnosti koja nedostaje mnogim dosadašnjim bazama podataka. Zaključno, vrijeme je da se današnje društvo suoči sa mnogim biološkim problemima kao što su izbjegavanje pandemija, očuvanje bioraznolikosti, pružanje biološke sigurnosti te zaštita vrsta.Barcoding associates taxonomy, molecular phylogenetics and population genetics. It demonstrates the possibility of creating a database of the animals on the basis of COI sequences. For other kingdoms of life it is necessary to conduct a more comprehensive analysis in order to find the most appropriate barcode. Further development and progress of this project will provide a clearer insight into the biodiversity, and analysis are much more objective than conventional morphological analysis. It is interesting to think of the existence of the online encyclopedia of life on Earth. Although the project is actually relatively novel, daily efforts are made to overcome any obstacles in the process of building this system. Methods in molecular biology are becoming cheaper and can be introduced into the process of barcoding. Development of many new methods such as pyrosequencing will allow rapid analysis of mixed samples. Techniques of nondestructive extraction of DNA from the recently collected samples are already developed, which promotes conservation of samples. All obstacles must be clearly defined and overridden so that BOLD database could develop fully and successfully. If an organization such as the Global Biodiversity Information Facility or All Species Foundation acquire this project, it would be a major step toward longevity lacking many current databases. Finally, it is time to confront today's society with many biological problems such as avoiding pandemics, biodiversity conservation, providing security and protection of biological species
