University of Zagreb. Faculty of Science. Department of Biology.
Abstract
Pauci, kao i ostali člankonošci, imaju otvoreni optjecajni sustav kroz koji teče njihova tjelesna tekućina – hemolimfa. Hemolimfa ima višestruku ulogu u kretanju, disanju i prehrani te zauzima otprilike 20% tjelesne mase pauka. U slučaju ozlijede dolazi do trenutnog gubitka hemolimfe ali i napada patogena što može rezultirati infekcijom. Imunološki sustav pauka lokaliziran je u hemocitima koji nastaju iz stanica stijenke miokarda u obliku prohemocita koji ulaze u hemolimfu i diferenciraju se u ostale tipove hemocita. Oni doprinose obrani od patogena fagocitozom, nodulacijom i enkapsulacijom invazivnih tijela. Cilj ovog rada je odrediti brojnost, pojedinačni udio i morfološka obilježja hemocita u hemolimfi pauka vrste Geolycosa vultuosa. Ovim radom utvrđeno je da metoda bojanja May-Grünwald giemsom najbolje omogućuje uvid u morfološka obilježja (dimenzije pojedinih tipova hemocita i pripadajućih jezgri, prisutnost vakuola, organela i granula) hemocita, što je značajni doprinos ovoj vrlo slabo opisanoj fiziologiji pauka. Poznavanje gore navedenih odrednica omogućilo bi lakše prepoznavanje pojedinih tipova hemocita u pauka općenito te olakšalo daljnja istraživanja na području hematologije i imunološkog odgovora u pauka.Spiders, as all other arthropods, have an open circulatory system, and their body fluid, the hemolymph, freely moves between lymphatic vessels and the body cavities. The hemolymph can be considered as a multifunctional organ, central for locomotion, respiration and nutrition, and it amounts to approximately 20 % of a spider’s body weight. Any injury includes not only immediate hemolymph loss but also pathogen attacks and subsequent infections. In spiders, the immune system is localized in hemocytes which are derived from the myocardium cells of the heart wall where they are produced as prohemocytes and from where they are released as different cell types into the hemolymph. They contribute to the defense against pathogens by phagocytosis, nodulation and encapsulation of invaders. The goal of this research is to consider the number and morphological characteristics of hemocytes of the spider species Geolycosa vultuosa. In this research the staining method May-Grünwald giemsa (MGG) was established as the best to describe morphological characteristics of hemocytes (cell size and their nuclei, presence of vacuoles, organels and granules) which is a significant contribution to the field of spider physiology that still lacks research. Data provided in this research will enable easier recognition of hemocyte types in spiders in general and further easier research in the field of spider hematology and their immune response
