Changes in the algal composition, bacterial metabolic activity and element content of biofilms developed on artificial substrata in the early phase of colonization

Abstract

Changes in the algal composition and metabolic profiles of bacterial communities as well as the inorganic components were studied on artificial substrata during the early phase of biofilm formation under laboratory conditions in September 2002 and 2003. Sterile Perspex and polished quartz glass discs with a diameter of 3 cm were placed into a Perspex rack, which was immersed vertically in an aquarium containing water from a shallow soda lake. The temperature was kept constant and sufficient oxygen supply was provided. The samples were illuminated for 12 hours a day. Periphyton communities were sampled from 2 to 126 hours of exposure. In both experiments, the alteration of the number of algal species and cells as well as the carbon source utilization of microbial communities was logarithmic. In the two years, considerable differences were revealed in the magnitude of algal cell numbers. The proportion of benthic and planktonic algae showed an undulating pattern in the second experiment. One of the dominant benthic species was the diatom Achnanthidium minutissimum Kütz., while that of the planktonic, the cyanobacterium Microcystis aeruginosa Kütz. During the experiments an increase in the bacterial activities could be observed; the higher the microbial diversity and abundance that was detected, the more BIOLOG carbon sources were utilized. The examined element contents indicated interactions among algae and bacteria in the biofilms from the beginning of the colonization processes