Katedra fyziky atmosféryDepartment of Atmospheric PhysicsFaculty of Mathematics and PhysicsMatematicko-fyzikální fakult

Dharam Singh (5123600)

Rakshak Kumar (125841)

Sanjay Kumar (8853)

Vishal Acharya (631470)

Standards in Genomic Sciences

Table S1. Whole genome sequence-based in silico comparison of strain ERGS5:01 and other related Janthinobacterium strains in database for DDH and ANI. (DOCX 17Â kb

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Additional file 2: of Strategies for high-altitude adaptation revealed from high-quality draft genome of non-violacein producing Janthinobacterium lividum ERGS5:01

Abstract A light pink coloured bacterial strain ERGS5:01 isolated from glacial stream water of Sikkim Himalaya was affiliated to Janthinobacterium lividum based on 16S rRNA gene sequence identity and phylogenetic clustering. Whole genome sequencing was performed for the strain to confirm its taxonomy as it lacked the typical violet pigmentation of the genus and also to decipher its survival strategy at the aquatic ecosystem of high elevation. The PacBio RSII sequencing generated genome of 5,168,928 bp with 4575 protein-coding genes and 118 RNA genes. Whole genome-based multilocus sequence analysis clustering, in silico DDH similarity value of 95.1% and, the ANI value of 99.25% established the identity of the strain ERGS5:01 (MCC 2953) as a non-violacein producing J. lividum. The genome comparisons across genus Janthinobacterium revealed an open pan-genome with the scope of the addition of new orthologous cluster to complete the genomic inventory. The genomic insight provided the genetic basis of freezing and frequent freeze-thaw cycle tolerance and, for industrially important enzymes. Extended insight into the genome provided clues of crucial genes associated with adaptation in the harsh aquatic ecosystem of high altitude

Rakshak Kumar

Vishal Acharya

Dharam Singh

Sanjay Kumar

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Strategies for high-altitude adaptation revealed from high-quality draft genome of non-violacein producing Janthinobacterium lividum ERGS5:01

Figure S3. Pan genome analysis of genus Janthinobacterium. The pan-genome profile plot displaying the total and the core gene families for each genome with a curve fit exponent of 0.43. [Number of unique genes observed for strains are displayed in brackets; 1. J. lividum ERGS5:01 (236); 2. J. sp. 551a (0); 3. J. agaricidamnosum DSM 9628 (1412); 4.J. sp. CG23_2 (2860); 5. J. sp. CG3(1038); 6. J. sp. HH01 (1764) 7.J. sp. KBS0711 (66) 8. J. lividum H-24 (217); 9. J. lividum NFR18 (86); 10. J. sp. Marseille (1625); 11. J. lividumMTR (197) 12.J. sp. OK676 (171); 13.J. lividum PAMC25724 (152); 14. J. psychotolerans S3–2 (644); 15.J. sp. RA13 (170); 16. J. lividum RIT308 (75); 17.J. sp. 344 (11); 18.J. sp. Ant5–2-1 (136); 19.J. sp. HH100 (15); 20.J. sp. HH102 (72); 21.J. sp._HH103 (16); 22.J. sp. HH104 (114); 23.J. sp. HH106 (100); 24.J. sp. HH107 (78); 25.J. sp. MP5059B (136); 26.J. sp. TND4EL3 (504); 27.J. sp. YR213 (92)]. (PDF 85 kb

Additional file 5: of Strategies for high-altitude adaptation revealed from high-quality draft genome of non-violacein producing Janthinobacterium lividum ERGS5:01

https://figshare.com/articles/Additional_file_2_of_Strategies_for_high-altitude_adaptation_revealed_from_high-quality_draft_genome_of_non-violacein_producing_Janthinobacterium_lividum_ERGS5_01/6164426

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