This is a software paper, which main objective is to provide practical information on how to use SPOT-GPR release 1.0, a MATLAB®-based software for the analysis of ground penetrating radar (GPR) profiles. The software allows detecting targets and estimating their position in a two-dimensional scenario, it has a graphical user interface and implements an innovative sub-array processing method. SPOT-GPR was developed in the framework of the COST Action TU1208 “Civil Engineering Applications of Ground Penetrating Radar” and is available for free download on the website of the Action (www.GPRadar.eu)