Tutul bamboo charcoal as carbon nanoparticle has various advantages in terms of physical and chemical properties. In this study, bamboo charcoal nanoparticle is produced using High Energy Ball Miling (HEBM) shaker mill model. Objective of this study is to characterize bamboo charcoal particles from result impact of Hihg Energy Ball Milling Shaker type, as a variable of empty space in the collector tube, whereas SEM and EDX to analyze particle diameter distribution and chemical composition contained in collision material. The cycle used is 2 million and 900 motor rpm. The steel ball diameter used is 1/4 inch. The tube is made of a stainless steel cylinder with a diameter of 2 inch and a length of 120 mm. The tube is filled with a ratio of 1:1, 1:2, 1:3, and 1:4. Result of the study shows that the variation of empty volume on the tube cannot be the main benchmark to determine bamboo charcoal particle size. The more empty space on tube volume, the more freely the ball movement as a grinder, but this situation makes bamboo charcoal bone to the edge of tube so that grinding with uneven steel balls. From SEM/EDX testing can be known the visualization of bamboo charcoal particle and its composition. From SEM photo visualization shows that average bamboo charcoal particle shape is not perfect and and the composition of the dominant chemical element is the element carbon reached 93.3