In this review, various aspect concerning the resolution of capillary electrophoresis for
nine standard peptides ( namely, bradykinin, bradykinin fragment 1-5, substance P,
Arg-vasopressin, luteinizing hormone·releasing hormone (LHRH), bombesin, leucine-enkephalin,
methionine-enkephalin and oxytocin). The experiment were carried out
using 100 mM phosphate buffer as the separation buffer, added with acetonitrile
(ACN) and triethylamine (TEA) as an additive at low pH. The resolution of these
peptides was examined at different concentrations of ACN ( 0, 10, 20, 30, 40, 50 and
60 %) and TEA ( 0, 5, 10, 20, 30, 40 ppm) at their respective final pH. The results
showed that the resolution was improve when 10 % of ACN added in the BGE. The
optimum resolution found at 40 % ACN where all peptides were resolved at baseline.
The shape was perfectly symmetrical. As for the TEA addition, adding 5 ppm already
improve the peaks and addition of 30 ppm TEA gives the optimum resolution
compare to all. For both ACN and TEA, adding more concentrated additive gives poor
resolution of peptides. The investigation was continued by applying voltage ( 23, 24,
25,26 and 27 kV). Shorter time separation achieved when voltage increased. The best
resolution found at 25 kV. However, more voltage caused poor resolution
