Trabajo presentado al: 4th International Meeting on Apicomplexa in Farm
Animals. (11-14 October 2017 - Madrid, Spain).Peer Reviewe

Benavides, Julio

Fernández Fernández, M.

García Sánchez, M.

Horcajo, P.

Jiménez Pelayo, L.

Ortega Mora, Luis M.

Osoro, Koldo

Regidor-Cerrillo, Javier

Rojo Montejo, S.

Román-Trufero, A.

Vázquez, P.

Fernández Fernández, Miguel

Digital.CSIC

 1                     APICOWPLEXA 2017 4th International Meeting on Apicomplexa in Farm Animals, 11-14 October 2017 - Madrid, Spain    ApiCOWplexa – El Escorial 2017       APICOWPLEXA 2017 4th International Meeting on Apicomplexa in Farm Animals, 11-14 October 2017 - Madrid, Spain   PROCEEDINGS        Complutense University of Madrid Euroforum, El Escorial (Madrid, Spain)ApiCOWplexa – El Escorial 2017 i   Scientific committee Alessandra Torina (Istituto Zooprofilattico Sperimentale della Sicilia "A.Mirri", Italy) Alexandre Leitao (University of Lisbon, Portugal) Andrew Hemphill (University of Berne, Switzerland) Brian Cooke (Monash University, Australia) Fiona Tomley (University of London, United Kingdom) Frank Katzer (Moredun Research Institute, United Kingdom) Gereon Schares (Friedrich-Loeffler-Institute, Germany) Jeroen Saeij (University of California-Davis, United States of America) Tülin Karagenç (Adnan Menderes University, Turkey) Wes Van Voorish (University of Washington, United States of America)   Organising committee Luis M. Ortega Mora (Universidad Complutense de Madrid) Ignacio Ferre Pérez (Universidad Complutense de Madrid) Gema Álvarez García (Universidad Complutense de Madrid) Esther Collantes Fernández (Universidad Complutense de Madrid) Javier Regidor Cerrillo (Universidad Complutense de Madrid) María Sonia Almería de la Merced (Universidad Autónoma de Barcelona) Julio Benavides Silván (Instituto de Ganadería de la Montaña) Marta González Warleta (Centro de Investigaciones Agrarias de Mabegondo) María Elvira Ares Maza (Universidad de Santiago de Compostela)   ApiCOWplexa – El Escorial 2017     Posters     104  Effects of challenge dose and inoculation route of virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid gestation  P 52  P. Vázquez1, K. Osoro2, M. Fernández3, A. Román-Trufero2, J. Regidor-Cerrillo1, L. Jiménez-Pelayo1, M. García-Sánchez1, S. Rojo-Montejo2, J. Benavides3, P. Horcajo1, L.M. Ortega-Mora1  1 Saluvet, Animal Health Department, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain 2 Regional Service for Research and Agri-Food Development (SERIDA), 33300 Villaviciosa, Asturias, Spain 3 Mountain Livestock Institute, Animal Health Department, University of León CSIC-ULE, 24346 Grulleros, León, Spain The standardization of bovine models remains as a challenging and high-priority issue to get a better understanding of immunopathological features occurring during Neospora caninum (NC) infection, but also to evaluate drugs and vaccines. Variables such as the stage of gestation, the parasite isolate, the challenge dose and the route of inoculation may lead to different patterns of foetal death presentation, as well as variable impact on parasite transmission rate. The aim of this study was to evaluate the effects of different challenge doses and routes of inoculation of the virulent Nc-Spain7 isolate on abortion and vertical transmission. For that, three doses of tachyzoites were inoculated intravenously (IV), and additionally subcutaneously (SC) for the mid dose, to 24 Asturiana pregnant heifers at day 110 of gestation (G1 (n=6): 107 IV, G2 (n=6): 105 IV, G3 (n=6): 105 SC, G4 (n=6): 103 IV and G5 (n=4): negative control). Foetal death (n=9) was observed in all infected groups; however, it occurred less frequently and later as dose decreased (G1: 66.7% (from 25 to 31 dpi); G2: 50.0% (from 29 to 39 dpi); G3: 16.7% (26 dpi); G4: 16.7% (38 dpi)). In terms of NC transmission, parasite DNA was found in the placental tissues of all abortion cases that could be examined. Lesions compatible with neosporosis were also confirmed in placental tissues from abortions of IV groups (7/7). All brain samples of fetuses from G1, G2 and G3 groups were positive for NC DNA (7/7) but only one brain (G1) resulted positive among calves (1/19). Indirect fluorescent antibody test was used for titration of thoracic fluids from aborted fetuses (n=6) and precolostral sera from calves (n=19). One aborted foetus from G2 at 39 dpi was positive at titre 1:16. Eighty percent of calves from G1 (2/2) and G2 (2/3) were seropositive, showing titres between 1:800 and 1:6400. Our results show that both dose and route of inoculation appear to influence clinical manifestations and NC transmission in experimental bovine neosporosis.       

English

Effects of challenge dose and inoculation  route of virulent Neospora caninum Nc-Spain7 isolate  in pregnant cattle at mid gestation

Effects of challenge dose and inoculation route of virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid gestation

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