Specificity of mAbs 1A4 and FB11.

Abstract

<p>Inactivated <i>F</i>. <i>tularensis</i> SCHU S4 (lane 1), SCHU S4 ΔwbtI (lacks <i>O</i>-antigen, lane 2), LVS (lane 3), <i>F</i>. <i>tularensis</i> subsp. <i>holarctica</i> (lane 4), subsp. <i>novicida</i> (lane 5), <i>F</i>. <i>philomiragia</i> (lane 6), purified <i>B</i>. <i>pseudomallei</i> LPS (lane 7) and <i>E</i>. <i>coli</i> LPS (lane 8) were separated on 12% SDS-PAGE gels and blotted onto nitrocellulose membranes. The membranes then were probed with mAbs 1A4 IgG3 (<b>Panel A</b>) and FB11 (<b>Panel B</b>). The existence of LPS in these samples was demonstrated by Pro-Q emerald 300 LPS staining (<b>Panel C</b>).</p