<p>A total of 3225 strains made up of 2997 strains from Bioneer ver2.0 library and 228 unique backcrossed strains from ver1.0 were screened. Each of the auxotrophic ver2.0 strains was serially diluted and manually spotted on 75 µg/ml DOXO and 116 strains were found to be repeatedly showing hypersensitivity on DOXO. These strains were backcrossed with prototrophic wild-type cells to remove all the nutrition marker mutations in the Bioneer strains in order to link the DOXO hypersensitive phenotypes to the indicated null mutations. 91 strains that showed hypersensitivity to various level of DOXO were obtained finally.</p
