<div><p>Migration and morphologies were compared for HT-1080s cultured in lower modulus synthetic ECM (140 Pa, 1500 μM CRGDS) and collagen (1.7 mg/mL, acid-extracted, rat tail collagen, BD Biosciences). Time-lapse microscopy (15 min / frame) illustrating HT-1080s migrating in (<b>A</b>) synthetic ECM (also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081689#pone.0081689.s026" target="_blank">Movie S15</a>) and (<b>B</b>) collagen (also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081689#pone.0081689.s020" target="_blank">Movie S9</a>). Scale bars = 25 μm.</p>
<p>HT-1080s migrated in lower modulus synthetic ECM and collagen with statistically equivalent (<b>C</b>) speed and (<b>D</b>) directionality (DTO/TD). <i>Box</i> and <i>whisker </i><i>plot </i><i>for </i><i>cell </i><i>speed</i>: White diamond = mean, white line = median, boxes = middle upper (top) and middle lower (bottom) quartile of the cell population, whiskers = highest (above) and lowest (below) migration speeds. Differences in HT-1080 morphology were determined by comparing quantified (<b>E</b>) circularity and (<b>F</b>) fraction of rounded cells (Elongation < 2.0). Circularity was calculated for individual cells (3 separate experiments, N > 200); Fraction of rounded cells was calculated per gel (at least two separate experiments, N ≥ 3 gels), with error bars representing standard deviation (*** = p < 0.001). </p></div
