<p>(a–c) Adult brains 3d and 30d <i>w</i><sup><i>1118</i></sup> flies, together with 30d<sup>Spd</sup> <i>w</i><sup><i>1118</i></sup> flies immunostained for Synapsin. Scale bar: 50 μm. (d) Quantification of Synapsin intensity within the central brain region normalized to 3d flies (<i>n</i> = 9–10 independent brains; Kruskal-Wallis test). (e–g) Adult brains of 3d and 30d <i>w</i><sup><i>1118</i></sup> flies, together with 30d<sup>Spd</sup> <i>w</i><sup><i>1118</i></sup> flies immunostained for Synaptotagmin-1 (Syt-1). Scale bar: 50 μm. (h) Quantification of signal intensity of Syt-1 in the central brain region normalized to 3d flies (<i>n</i> = 8–9 independent brains; Kruskal-Wallis test with Dunn’s multiple comparison test, <i>p</i>-values were subject to Bonferroni correction). (i–k) Adult brains of 3d, 30d <i>w</i><sup><i>1118</i></sup>, and 30d<sup>Spd</sup> <i>w</i><sup><i>1118</i></sup> flies immunostained for BRP (using Nc82 and N-terminal antibodies) and RBP. Scale bar: 50 μm (l–n) Quantification of BRP (using Nc82 and N-terminal antibodies) and RBP intensities within the central brain region normalized to 3d flies (<i>n</i> = 14–18 independent brains; Kruskal-Wallis test with Dunn’s multiple comparison test, <i>p</i>-values were subject to Bonferroni correction). ** <i>p</i> < 0.01, *** <i>p</i> < 0.001, ns = not significant, <i>p</i> ≥ 0.05. Underlying data is shown in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002563#pbio.1002563.s001" target="_blank">S1 Data</a>.</p
