Bahl, Martin Iain

Bay Gosewinkel, Ulrich

Bester, Kai

Bohse Hendriksen, Niels

Escola Casas, Monica

Frandsen, Henrik Lauritz

Licht, Tine Rask

Nielsen, Lene Nørby

Roager, Henrik Munch

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Apr 10, 2018Do pesticides affect the intestinal bacterial community and does this have healthimplications?Nielsen, Lene Nørby; Roager, Henrik Munch; Escola Casas, Monica; Frandsen, Henrik Lauritz; BayGosewinkel, Ulrich; Bester, Kai; Licht, Tine Rask; Bohse Hendriksen, Niels; Bahl, Martin IainPublication date:2017Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Nielsen, L. N., Roager, H. M., Escola Casas, M., Frandsen, H. L., Bay Gosewinkel, U., Bester, K., ... Bahl, M. I.(2017). Do pesticides affect the intestinal bacterial community and does this have health implications?. Abstractfrom Dansk Selskab for Toksikologi og Farmakologi Årsmøde, Sønderborg, Denmark.    September 11, 2017    Members of the Board  Eva C. Bonefeld-Jørgensen President  Gunnar Toft Treasurer, Section for Toxicology    Agnete Larsen Secretary,  Section for Pharmacology  Lisbeth E. Knudsen Section for Toxicology  Anders Jensen Section for Pharmacology     Homepage www.dsftm.dk  Contact info@dsftm.dk  Tax Reg: 33976771    Annual Meeting, Sandbjerg Manor, Sønderborg 2-3. November 2017 “Gut, Metabolism and Brain in Health and disease”  Abstract Title Do pesticides affect the intestinal bacterial community and does this have health implications? Author’s (underline the presenting author) Lene Nørby Nielsena, Henrik M. Roagera, Mònica Escolà Casasb, Henrik L. Frandsena, Ulrich Gosewinkelb, Kai Besterb, Tine Rask Lichta, Niels Bohse Hendriksenb and Martin Iain Bahla Institutions aNational Food Institute, Technical University of Denmark bDepartment of Environmental Science, Aarhus University  Background The intestinal bacterial community is now recognized as an important factor for health and implicated in numerous states of disease including, but not limited to, inflammatory bowel diseases. Despite the fairly extensive regulatory demands for risk assessment of pesticides in relation to human exposure, there is currently very little knowledge related to potential effects on the gut microbiota. It has however recently been speculated that glyphosate based herbicides may affect the gut microbiota of humans and animal husbandry due to inhibition of the Shikimate pathway in bacteria causing loss of aromatic amino acid synthesis and growth inhibition, supported by in vitro studies. Here we present results from the first animal trial to address this question. Methods Sprague Dawley rats (n=20/group) were exposed to glyphosate at 5x and 50x the acceptable daily intake (ADI) for humans. 16S rRNA gene sequencing of the bacterial community and liquid chromatography mass spectrometry (LC-MS) based metabolic profiling of aromatic amino acids and their downstream metabolites was applied to intestinal samples obtained after two weeks of oral dossing. Results We found that glyphosate had very limited effects on bacterial community composition even at the highest exposure concentration. Also we find relatively high concentrations of aromatic amino acids in the intestine of the animals.  Discussion and conclusion Our data show that glyphosate inhibits bacterial growth in minimal medium but this inhibitory effect is relieved in the presence of aromatic amino acids in the growth medium. Results from the animal trial suggest that sufficient levels of aromatic amino acids are present in the rat intestine to alleviate the need for bacterial synthesis and thus prevent an antimicrobial effect of glyphosate in vivo.  

Do pesticides affect the intestinal bacterial community and does this have health implications?

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Do pesticides affect the intestinal bacterial community and does this have health implications?

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