Immediate
hemorrhage control and anti-infection play important roles in the
wound management. Besides, a moist environment is also beneficial
for wound healing. Hydrogels are promising materials in urgent hemostasis
and drug release. However, hydrogels have the disadvantage of rapid
release profiles, leading to the exposure to high drug concentrations.
In this study, we constructed hybrid hydrogels with rapid hemostasis
and sustainable antibacterial property combining aminoethyl methacrylate
hyaluronic acid (HA-AEMA) and methacrylated methoxy polyethylene glycol
(mPEG-MA) hybrid hydrogels and chlorhexidine diacetate (CHX)-loaded
nanogels. The CHX-loaded nanogels (CLNs) were prepared by the enzyme
degradation of CHX-loaded lysine-based hydrogels. The HA-AEMA and
mPEG-MA hybrid hydrogel loaded with CLNs (labeled as Gel@CLN) displayed
a three-dimensional microporous structure and exhibited excellent
swelling, mechanical property, and low cytotoxicity. The Gel@CLN hydrogel
showed a prolonged release period of CHX over 240 h and the antibacterial
property over 10 days. The hemostasis and wound-healing properties
were evaluated in vivo using a mouse model. The results showed that
hydrogel had the rapid hemostasis capacity and accelerated wound healing.
In summary, CLN-loaded hydrogels may be excellent candidates as hemostasis
and anti-infection materials for the wound dressing application
