Additional file 8: Fig. S6. Amplification of spalt from cDNA extracted from zygotes and 24-h post-fertilization Crepidula embryos. Amplification of spalt (Cfo-spalt) was only detected using cDNA from 24-h post-fertilization Crepidula embryos. There was no amplification at all when cDNA from zygotes was used. The gene twist from Crepidula (Cfo-twist) was used as a positive control since twist is detected at both stages of development. PCR products were run on 1% agarose gels
