University of Zagreb. Faculty of Science. Department of Biology.
Abstract
Rekombinantna DNA tehnologija oblikovanje je novih kombinacija nasljednog materijala koje se dobivaju ugradnjom molekula nukleinskih kiselina dobivenih izvan stanice putem virusa, plazmida ili bilo kojeg drugog oblika prenositelja. Time se omogućava njihova ugradnja u organizam domaćina u kojem one prirodno ne postoje ali u kojem su sposobne za umnožavanje. Naime, tradicionalnim biotehnološkim metodama (selekcija, križanje itd.) već se stoljećima nastoje unaprijediti svojstva biljaka i životinja koje se koriste za proizvodnju hrane, ili poboljšati i prilagoditi prehrambene proizvode (mikroorganizmi, kvasci, fermentacija itd.). Isto se nastoji i genetičkim inženjerstvom, međutim, njime se kreiraju, poboljšavaju ili modificiraju biljke, životinje i mikroorganizmi izmjenom genskog materijala bez barijera vrste, odnosno među nesrodnim vrstama, što je bitna razlika u odnosu na tradicionalne biotehnološke metode.Recombinant DNA technology is forming the new combination of hereditary material that comes with fitting nucleic acid molecules obtained outside the cells via viruses, plasmids, or any other form of transmitter. This allows their incorporation into the host organism in which they naturally do not exist but where they are capable of reproduction. Specifically, traditional biotechnology methods (selection, crossing etc.) are already for centuries seeking to improve the properties of plants and animals used for food production, or enhance and customize nutritional products (microorganisms, yeasts, fermentation, etc.). The same is attempted with genetic engineering, however, it does create, improve or modify plants, animals and microorganisms without changing the genetic material of a barrier type, or between unrelated species, which is a big difference compared to the traditional biotechnology methods