The aim of this study was to determine the 96h LC50 of copper and cadmium, and also, to evaluate the effects of their sub-lethal dose on stress factors in Persian sturgeon, Acipenser persicus. Obtained results from Probit analysis showed 96h LC50 values of 0.502 and 14.78mg/l for copper and cadmium, respectively. Also, in single sub-lethal exposures, 72 juveniles (130±19g) were exposed to 0.026 and 0.68mg/l of copper and cadmium in semi- static conditions, and some stress-related biochemical factors were assessed in 1, 7 and 14 days. According to the obtained results, plasma glucose and cortisol were increased (P0.05) differences in plasma and liver protein contents between experimental groups and controls with the exception of copper treatment at the first day of sampling when plasma protein contents showed significant increases, but decreased significantly (P<0.05) in the subsequent sampling stages. In addition, with copper treatment, significant decreases were observed in plasma triglyceride concentrations as time passed by compared to day 1 (P<0.05). Results showed that copper is more toxic than cadmium for this species and also, 96h LC50 of copper and cadmium are stressful for Persian sturgeon

Hedayati, M.

Makhdoomi, Ch.

Mirvaghefi, A.R.

Mojazi Amiri, B.

Rafiee, Gh.R.

Zahedi, S.

Zarei Dengasraki, M.

Aquatic Commons

  
Acipenser persicus
szahedit@gmail.com
LC50
Acipenser persicus
LC50
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Gravel et al., 2005
Dethloff et al., 1999
Witeska, 2005
Sanchez et al., 2005
Romeo et al., 2000
Miliou et al., 1998
Cinier et al., 1997; Kim et al., 
2004; Asagba et al., 2008
Brucka-Jastrzebska & Protawicki, 2005
Lizardo-dault et al., 2007
Pratap & Wendelaar Bonga, 1990; Hontela et al.,) 
1996Reid & McDonald,) 
(1988
Agusa et al., 
2004
De Mora et al., 2004; Saeedi & Karbassi, 
2006; Parizanganeh et al., 2008
Karpinskey, 1992Ikemoto et al., 2004
Acipenser persicus
Khodorevskaya et 
al., 1997
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LC50Mirzaei et al., 
2004LC50
LC50
Karan et al., 1998; Hansen et al., 
200096 LC50
(Haddadi Moghadam et al., 2009)
Cicik & Engin, 2005
Oreochromis mosambicus
Wendlaar Bonga, 1990)&Pratap
Canli, 1995
Singh & Sharma, 1998Almeida
De la Torre 
et al., 2000
Umminger, 1970
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Wu et 
al., 2007
Oreochromis mossambicusPratap & Wendelaar 
Bonga, 1990Tort et al., 1996
Veillette & Yong, 2004
Robert
Ricard 
Wu et al., 
2007
pH 
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Agusa T., Kunito T., Tanabe S., Pourkazemi M. 
and Aubrey D.G., 2004. Concentrations of 
trace elements in muscle of sturgeons in the 
Caspian Sea. Marine Pollution Bulletin, 
49:789 800. 
Almeida J.A., Novelli E.L.B., Silva M.D. and 
Alves Junior R., 2001. Environmental 
cadmium exposure and metabolic responses of 
the Nile tilapia, Oreochromis niloticus. 
Environmental Pollution, 21:169-175
Asagba S.O., Eriyamremu G.E. and Igberaese 
M.E., 2008. Bioaccumulation of cadmium and 
its biochemical effects on selected tissues of 
the catfish (Clarias gariepinus). Fish 
Physiology and Biochemistry, 34:61-69. 
Brucka-Jastrzebska E. and Protawicki M., 2005. 
Effects of cadmium and nickel exposure on 
haematologocal parameters of common carp, 
Cyprinus carpio. Acta Ichthyology et 
Piscatoria, 35(1):29-38
Canli M., 1995. Effects of mercury, chromium and 
nickel on some blood parameters in the carp, 
Cyprinus carpio. Turkish Journal of Zoology, 
19:305-311. 
Canli M., Ay O. and Kalay M., 1998. Levels of 
heavy metals (Cd2+, Pb2+, Cu2+, Cr2+ and Ni2+) 
in tissue of Cyprinus carpio, Barbus Capito 
and Chondrostoma regium from the Seyhan 
River. Turkish Journal of Zoology, 22:149-157
Charkhabi A.H., Sakizadeh M. and Rafiee G., 
2005. Seasonal fluctuation of heavy metals 
pollution in Iran's Siahrood River. 
Environmental Science and Pollution Research, 
12:264 270. 
Cicik B. and Engin K., 2005. The effects of 
cadmium on levels of glucose in serum and 
glycogen reserves in the liver and muscle 
tissues of Cyprinus carpio. Turkish Journal of 
Veterinary and Animal Sciences, 29:113-117
Cinier C., Petit-Ramel M., Faure R., Garin D. 
and Bouvet Y., 1999. Kinetics of cadmium 
accumulation and elimination in carp, Cyprinus 
carpio tissue. Comparative Biochemistry and 
Physiology, 122C:345-352. 
De la Torre F.R., Salibian A. and Ferrari L., 2000. 
Biomarkrs assessment in juvenile Cyprinus carpio 
exposed to waterborne cadmium. Environmental 
Pollution, 109:277-282
De Mora S., Sheikholeslami M.R., Wyse E., Azemard 
S. and Cassi R., 2004. An assessment of metal 
contamination in coastal sediments of the Caspian 
Sea. Marine Pollution Bulletin, 48:61 77. 
Dethloff G.M., Schlenk D., Khan S. and Bailey 
H.C., 1999. The effects of copper on blood and 
biochemical parameters of rainbow trout 
(Oncorhynchus mykiss). Archive of Environ-
mental Contamination and Toxicology, 36:415-
423. 
Dumont H.J., 1998. The Caspian Lake: History, 
biota, structure and function. Limnology and 
Oceanography, 43:44-52
Gopal V., Parvathy S. and Balasubramanian 
P.B., 1997. Effects of heavy metals on the 
blood protein biochemistry on the fish 
Cyprinus carpio and its use as a bio-andicator 
of pollution stress. Environmental Monitoring 
and Assessment, 48:117-124
Gravel A., Campell P.G.C. and Hontela A., 2005. 
Distruption the hypothalamo-pituitary-inter-
D
ow
nl
oa
de
d 
fro
m
 is
fj.i
r a
t 1
7:3
7 +
03
30
 on
 M
on
da
y N
ov
em
be
r 2
7th
 20
17
    
    
  [ 
DO
I: 1
0.2
20
92
/IS
FJ
.20
17
.10
99
92
 ]  
  
renal axis in 1+ yellow perch (Perca flavescens) 
chronically exposed to metals in the 
environment. Canadian Journal of Fisheries 
and Aquatic Sciences, 62:982-990
Haddadi Moghadam K., Pajand Z., Parandavar 
H., Chubian F. and Pahlavan Yali M., 2009. 
Diet composition of the Persian sturgeon 
Acipenser persicus at the coast of the Guilan 
province, Caspian Sea. Russian Journal of 
Marine Biology, 35(4):331 334. 
Hansen J.A., Lipton J. and Welsh P.G., 2000. 
Acute responses of Bull trout (Salvelinus 
confluentus) to cadmium, copper and zinc. In: 
Third Society of Environmental Toxicology 
and Chemistry Congress, 10th  Annual Meeting 
of SETACEurope, Brighton, UK, May 21 25. 
Hontela A., Daniel C. and Ricard A.C., 1996. 
Effects of acute and subacute exposures to 
cadmium on the interrenal and thyroid function 
in rainbow trout, Oncorhynchus mykiss. 
Aquatic Toxicology. 35:171-182. 
Humtsoe N., Davoodi R., Kulkarni B.G. and 
Chavan B., 2007. Effect of arsenic on the 
enzymes of the Rohu carp, Labio rohita. The 
Raffles Bulletin of Zoology, 14:17-19
Ikemoto T., Kunito T., Watanabe I., Yasunaga 
G., Baba N., Miyazaki N., Petrov, E.A. and 
Tanabe S., 2004. Comparison of trace element 
accumulation in Baikal seals (Pusa sibirica), 
Caspian seals (Pusa caspica) and northern fur 
seals (Callorhinus ursinus). Environmental 
Pollution,127:83 97. 
Karan V., Vitoric V., Tutanclyc V. and Poleksic 
V., 1998. Functional enzymes activity and gill 
histology of carp after copper sulfate exposure 
and recovery. Ecotoxicology and Environ-
mental Safety, 40:49-55. 
Karpinskey M.G., 1992. Aspects of the Caspian 
Sea benthic ecosystem. Marine Pollution 
Bulletin, 24:389-394
Khodorevskaya R.P., Dovgopol G.F., Zhuravleva 
O.L. and Vlasenko A.D., 1997. Present status 
of commercial stocks of sturgeons in the 
Caspian Sea basin. Environmental Biology of 
Fishes, 48:209 219. 
Kim S., Jee J. and Kang J., 2004. Cadmium 
accumulation and elimination in tissues of 
juvenile olive flounder, Paralichthys olivaceus 
after sub-chronic cadmium exposure. 
Environmental Pollution,127:117-123. 
Lizardo-Dault H.M., Bains O.S., Singh C.R. and 
Kennedy C.J., 2007. Biosynthetic capacity of 
rainbow trout (Oncorhynchus mykiss) 
interrenal tissue after cadmium exposure. 
Archives of Environmental Contamination and 
Toxicology, 52:66-90
Miliou H., Zaboukas N. and Moraitou-
Apostolopoulou M., 1998. Biochemical 
composition, growth and survival of the guppy, 
Poecilia reticulate, during chronic sub-lethal 
exposure to calcium. Archives Environmental 
Contamination and Toxicology,35:58-63
Mirzaei J., Nezami S., Mehdinejad K., Pajand 
Z.O. and Alinejad R., 2004. Acute toxicity 
(96-h LC50) of heavy metals (Pb, Zn, Cu and 
Cd) in two species of sturgeon (Acipenser 
persicus and Acipenser stellatus). In: 5th  
Symposium of Sturgeon Fish. Ramsar, Iran. 
GB12
D
ow
nl
oa
de
d 
fro
m
 is
fj.i
r a
t 1
7:3
7 +
03
30
 on
 M
on
da
y N
ov
em
be
r 2
7th
 20
17
    
    
  [ 
DO
I: 1
0.2
20
92
/IS
FJ
.20
17
.10
99
92
 ]  
  
OECD, 1992. OECD guideline for testing of 
chemicals. Section 2, No. 203. Fish acute 
toxicity test, adopted July 17. 
Parizangane A., Lakhan V.C., Jalalian H. and 
Ahmad S.R., 2008. Contamination of near shore 
surficial sediments from the Iranian coast of 
Caspian Sea. Soil and Sediment Contamination, 
17:19-28
Pratap H.B. and Wendlaar Bonga S.E.W., 1990. 
Effects of water-borne cadmium on plasma 
cortosol and glucose in the cichlid fish, 
Oreochromis mossambicus. Comparative 
Biochemistry and Physiology, 95C:313-317
Reid S.D. and McDonald D.G., 1988. Effects of 
Cd, Cu, and low pH on ion fluxes in the 
rainbow trout, Salmo gairdneri. Canadian 
Journal of Fishieries and Aquatic Sciences, 
45:244-253. 
Ricard A.C., Daniel C. and Holenta A., 1998. 
Effects of subchronic exposure to cadmium 
chloride on endocrine and metabolic functions 
in rainbow trout, Oncorhynchus mykiss. 
Archives of Environmental Contamination and 
Toxicology, 34:377-381
Robert M., Sapolsky M., Romeo M.L. and Munck 
A.U., 2000. How do glucocorticoids influence 
stress responses integrating permissive, 
suppressive, stimulatory, and preparative actions. 
Endocrin Reviews, 21: 55-80. 
Romeo M., Bennani N., Gnassia-Barelli M., 
Lafaurie M. and Girard J.P., 2000. Cadmium 
and copper display different responses towards 
oxidative stress in the kidney of the sea bass, 
Dicentrarchus labrax. Aquatic Toxicology, 
48:185-194
Saeedi M. and Karbassi A., 2006. Heavy metal 
pollution and speciation in sediments of 
southern part of Caspian Sea. Pakistani Journal  
of Biology Sciences, 9(4):733-740
Sanchez W., Palluel O., Meunier L., Coquery M., 
Porcher J. and  Ait- Aisa S., 2005. Copper-
induced oxidative stress in the three-spined 
stickleback: Relationship with hepatic metal levels. 
Environmental Toxicology and Pharmacology, 
19:177-183
Singh R.K. and Sharma B., 1998. Carbofuran 
induced biochemical changes in Claria 
batrachus. Pesticide Science, 53:285-290. 
Sokal R.R. and Rohlf F.J., 1995. Biometry: The 
principles and practice of statistics in biological 
research. 3rd  ed. W.H. Freeman and Company, 
New York, USA. 887P. 
Tort L., Kargacin B., Torres P., Giralt M. and 
Hidalgo J., 1996. The effect of cadmium 
exposure and stress on plasma cortisol, 
metallothionein levels and oxidative status in 
rainbow trout (Oncorhynchus mykiss) liver. 
Comparative Biochemistry and Physiology, 
114:29-34. 
Umminger B.L., 1970. Physiological studies on 
super cooled fish Fundulus heteroclitus. 
Carbohydrate metabolism and survival at sub 
zero temperature, Journal of Experimental 
Zoology, 17:159 174. 
Veillette P.A. and Young G., 2004. Temporal changes 
in intestinal Na+, K+-ATPase activity and in vitro 
responsiveness to cortisol in juvenile Chinook 
salmon. Comparative Biochemistry and 
Physiology, 138A:297 303. 
D
ow
nl
oa
de
d 
fro
m
 is
fj.i
r a
t 1
7:3
7 +
03
30
 on
 M
on
da
y N
ov
em
be
r 2
7th
 20
17
    
    
  [ 
DO
I: 1
0.2
20
92
/IS
FJ
.20
17
.10
99
92
 ]  
  
Watanabe I., Kunito T., Tanabe S., Amano M., 
Koyama Y., Miyazaki N., Petrov E.A. and 
Tatsukawa R., 2002. Accumulation of heavy 
metals in Caspian seals (Phoca caspica). 
Archive of Environmental Contamination and 
Toxicology, 43:109 120. 
Witeska M., 2005. Stress in fish-hematological and 
immunological effects of heavy metals. 
Electronic Journal of Ichthyology, 1:34-41. 
Wu S.M., Shih M.J. and Ho Y.C., 2007. 
Toxicological stress responses and cadmium 
distribution in hybrid tilapia (Oreochromis spp) 
upon cadmium exposure. Comparative 
Biochemistry and Physiology, 145C:218-226. 
D
ow
nl
oa
de
d 
fro
m
 is
fj.i
r a
t 1
7:3
7 +
03
30
 on
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on
da
y N
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em
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7th
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I: 1
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Iranian Scientific Fisheries Journal                                               Vol. 20, No. 2, Summer 2011 
  
The effects of exposure to sub-lethal copper and cadmium 
concentrations on biochemical factors of one year old  
Persian sturgeon, Acipenser persicus 
Zahedi S.(1)*; Mirvaghefi A.R.(2); Rafiee Gh.R.(3); Mojazi Amiri B.(4); 
Hedayati M.(5); Makhdoomi Ch.(6) and Zarei Dengasraki M.(7) 
szahedit@gmail.com 
1,2,3,4 Faculty of Natural Resources, Tehran University, P.O.Box: 4111 Karaj, Iran 
5- Research Institute for endocrine Sciences, Shahid Beheshti University of Medical Sciences 
6,7- Shahid Rajaee Fishes Rearing Center, P.O.BoX: 833  Sari, Iran 
Received: August 2010                          Accepted: June 2011  
Keywords:  Pollutants, Pollution, Cortisol, Acipenser persicus 
Abstract 
The aim of this study was to determine the 96h LC50 of copper and cadmium, and also, to 
evaluate the effects of their sub-lethal dose on stress factors in Persian sturgeon, Acipenser 
persicus. Obtained results from Probit analysis showed 96h LC50 values of 0.502 and 
14.78mg/l for copper and cadmium, respectively. Also, in single sub-lethal exposures, 72 
juveniles (130±19g) were exposed to 0.026 and 0.68mg/l of copper and cadmium in semi-
static conditions, and some stress-related biochemical factors were assessed in 1, 7 and 14 
days. According to the obtained results, plasma glucose and cortisol were increased (P<0.05) 
in experimental fishes compared to the controls only in the first day of sampling. There were 
no significant (P>0.05) differences in plasma and liver protein contents between 
experimental groups and controls with the exception of copper treatment at the first day of 
sampling when plasma protein contents showed significant increases, but decreased 
significantly (P<0.05) in the subsequent sampling stages. In addition, with copper treatment, 
significant decreases were observed in plasma triglyceride concentrations as time passed by 
compared to day 1 (P<0.05). Results showed that copper is more toxic than cadmium for this 
species and also, 96h LC50 of copper and cadmium are stressful for Persian sturgeon. 
*Corresponding author 
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The effects of exposure to sub-lethal copper and cadmium concentrations on biochemical factors of one year old Persian sturgeon, Acipenser persicus

http://aquaticcommons.org/21835/1/ISFJ-v20n2p61-fa.pdf

The effects of exposure to sub-lethal copper and cadmium concentrations on biochemical factors of one year old Persian sturgeon, Acipenser persicus

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