The aim of the present study was to investigate the effects of dietary oligofructose (1, 2 and 3%) on blood profiles of beluga Huso huso juveniles (18.77±0.76g). After 7 weeks feeding on experimental diets hematological parameters, metabolic products (cholesterol, glucose and total protein) and serum enzymes (lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) were measured. Compared to the control group (0% oligfructose), oligofructose had no effects on red blood cell counts (RBC), mean corposcular volume (MCV), mean cellular hemoglobin concentration (MCH) or mean cell hemoglobin concentration (MCHC) (P>0.05). However, hematocrit (Hct), hemoglobin concentration (Hb) and leucocyte counts (WBC) were significantly higher in fish fed 2% oligofructose (P0.05). These results indicate an immunomodulatory effect and the potential to reduce serum cholesterol levels in beluga sturgeon

Darvish Bastami, K.

Hoseinifar, S.H.

Khoshbavar Rostami, H.A.

Mirvaghefi, A.R.

Mojazi Amiri, B.

Aquatic Commons

  
Huso huso Linnaeus, 1758
Hoseinifar@ut.ac.ir
Huso huso
MCVMCH
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Gibson et al. 2004
Acipenser baeriMahious & Ollevier, 
2005Clarias gariepinusMahious & 
Ollevier, 2005Psetta maximaMahious et 
al., 2006Fenneropenaeus indicus
Hoseinifar et al., 2010
Hoseinifar et al., 2010b
Asadi et al., 
2006 ;Houston, 1997
Irianto & 
;Austin, 2002Brunt & Austin, 2005
Cerezuela
Spaus 
aurata
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Ninees, 1999
Mahious & Ollevier, 2005Orafti
Raffinerie Tirlemontoise, Tienen, Belgium
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MCHC
Biuretcholesterol oxidase 
glucose oxidase
Auto analyserEppendorf, 
EPOS, GermanyPars Azmoon,Tehran, 
IranShahsavani et al., 2008
WTW
Oximeter, oxi320/set wtwpH
PH 323- B/setl-wtw.Best-Nr100745
Cond 330i/set wtw
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testZar, 1994
SPSS
Excel, 2007
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Iranian Scientific Fisheries Journal                                                             Vol. 20, No. 2, Summer 2011 
  
The effects of prebiotic oligofructose on hematological, serum 
biochemical parameters and liver enzymes of juvenile beluga 
(Huso huso)  
Hoseinifar S.H.(1)*;  Mirvaghefi A.R.(2); Mojazi Amiri B.(3);  
Khoshbavar Rostami H.A.(4) and Darvish Bastami K.(5) 
Hoseinnfar@ut.ac.ir 
1,2,3-Faculty of  Natural Resources, University of Tehran, P.O.Box: 4111 Karaj, Iran 
4-Inland Waters Aquatics Stocks Research Center, P.O.Box: 139 Gorgan, Iran 
5-Iranian National Institute for Oceanography (INCO), P.O.Box: 14118-13389 Tehran, Iran 
Received: July 2010                          Accepted: December  2010  
Keywords: Prebiotic, Oligofructose, Hematology parameters, Serum enzymes, Beluga  
Abstract 
The aim of the present study was to investigate the effects of dietary oligofructose (1, 2 and 
3%) on blood profiles of beluga Huso huso juveniles (18.77±0.76g). After 7 weeks feeding on 
experimental diets hematological parameters, metabolic products (cholesterol, glucose and 
total protein) and serum enzymes (lactate dehydrogenase, alkaline phosphatase, alanine 
aminotransferase and aspartate aminotransferase) were measured. Compared to the control 
group (0% oligfructose), oligofructose had no effects on red blood cell counts (RBC), mean 
corposcular volume (MCV), mean cellular hemoglobin concentration (MCH) or mean cell 
hemoglobin concentration (MCHC) (P>0.05). However, hematocrit (Hct), hemoglobin 
concentration (Hb) and leucocyte counts (WBC) were significantly higher in fish fed 2% 
oligofructose (P<0.05). Although serum glucose and total protein remained unaffected, 
cholesterol was significantly lower in the 2% oligofructose group (P<0.05). Our results 
showed oligofructose had no significant effects on serum lactate dehydrogenase, alkaline 
phosphatase, alanine aminotransferase and aspartate aminotransferase (P>0.05). These results 
indicate an immunomodulatory effect and the potential to reduce serum cholesterol levels in 
beluga sturgeon.  
*Corresponding author 
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The effects of prebiotic oligofructose on hematological, serum biochemical parameters and liver enzymes of juvenile beluga (Huso huso)

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The effects of prebiotic oligofructose on hematological, serum biochemical parameters and liver enzymes of juvenile beluga (Huso huso)

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