Ingmer, H.

Klaas, I.

Mahmmod, Y.

Pedersen, K.

Svennesen, L.

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Downloaded from orbit.dtu.dk on: Apr 10, 2018Coagulase negative staphylococci distribution in dairy herds with automatic milkingsystem and their crosstalk with Staphylococcus aureus from IMI and teat apexMahmmod, Y.; Klaas, I.; Svennesen, L.; Pedersen, Karl; Ingmer, H.Publication date:2018Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Mahmmod, Y., Klaas, I., Svennesen, L., Pedersen, K., & Ingmer, H. (2018). Coagulase negative staphylococcidistribution in dairy herds with automatic milking system and their crosstalk with Staphylococcus aureus from IMIand teat apex. Poster session presented at National Mastitis Council Annual Meeting, Tucson, United States.Coagulase Negative Staphylococci Distribution in Dairy Herds with Automatic Milking System and their Crosstalk with Staphylococcus Aureus from IMI and Teat ApexMahmmod Y1, Klaas I1,2, Svennesen L1, Pedersen K3 , Ingmer H11 University of Copenhagen, Frederiksberg C, Denmark2 DeLaval International AB, Tumba, Sweden (present)3 Technical University of Denmark, Kongens Lyngby, DenmarkCNS species Sample type (%) P-valueMilk (n=105) Teat (n=268)S. arlettae (12) 1 (0.9) 11 (4.1) 0.22 NSS. capitis (3) --- 3 (1.1) NA*S. chromogenes (16) 11 (10.5) 5 (1.9) 0.002***S. cohnii (43) 5 (4.8) 38 (14.2) 0.011**S. epidermidis (60) 52 (49.5) 8 (3.0) <0.0001 ****S. equorum (122) 6 (5.7) 116 (43.3) <0.0001 ****S. haemolyticus (58) 16 (15.2) 42 (15.7) 0.90 NSS. hominis (17) 3 (2.9) 14 (5.2) 0.48 NSS. piscifermentans (2) --- 2 (0.8) NA*S. saprophyticus (5) --- 5 (1.9) 0.36 NSS. sciuri (9) --- 9 (3.4) 0.13 NSS. simulans (2) 2 (1.9) --- NA*S. succinus (2) --- 2 (0.8) NA*S. vitulinus (1) --- 1 (0.4) NA*S. warneri (2) 1 (0.9) 1 (0.4) NA*S. xylosus (19) 8 (7.6) 11 (4.1) 0.26 NSRESULTS 80% (228/284) quarters (142 cows) had ≥ 1 CNS species. 373 isolates, milk (n=105) and teat skin (n=268). 16 CNS species: teat skin (n=15) , milk (n=10) Coinfections (mixed): 11 quarters from milk samples and 66quarters from teat samples. Downregulation of S.aureus by some CNS may be explainedby its ability to inhibit S. aureus agr system throughproduction of auto-inducing peptide (AIP) molecules. Table 1 shows CNS species distribution and Figure 1 showscrosstalk between S. aureus and CNSTable 1. Species distribution and association of CNS isolates from aseptic quarter milk and teat skin samples collected from 142 cows CONCLUSIONS S. epidermidis and S. chromogenes are milk-associated, while S. equorum and S. cohnii are teat-associated.  CNS species, habitat type, and herd factors affect CNS and S. aureus crosstalk patterns.  Downregulation of S.aureus by some CNS species could explain possible protective effect.Figure 1. Crosstalk patterns between S. aureus and CNSyasser@sund.ku.dkDownregulation effect62, 65: S. arlettae (teat, H7)61: S. chromogenes (teat, H7)5: S. chromogenes (milk, H3)Variant effect1: S.equorum (milk, H1)2:  S.equorum (milk, H2)6: S.epidermidis (milk,H3)58: S.epidermidis (teat,H7)No effect66: S. Sciuri (teat, H7)7: S. Sciuri (teat, H3)METHODOLOGY Herd selection: 8 herds: AMS with ≥3 milking robots and bulk tank PCR Ct-values ≤32 for S. agalactiae Cows selection: 30-40 cows/herd: SCC: ≥ 200,000 cells/mL, and no antibiotic < 4 weeks prior to sampling  Quarter selection: RH & LF quarters of cows with odd lab number (1,3,5..)  Teat skin swabs (modified wet-dry method) and aseptic milk samples collected for bacterial culture using calf blood agar and SA SELECT™  Suspicious CNS colonies were subjected to MALDI-TOF assay for species identification. Interaction between S. aureus and CNS investigated with Qualitative Beta-Galactosidase Reporter Plate Assay based on 3 reporter strains of S. aureus ; hla (a-hemolysin),  RNAIII(key effector molecule of agr) and spa (Protein A). BACKGROUND  Longer milking duration and frequent exposure to teat disinfectants may affect the teat apex microbiota.  Knowledge of epidemiological characteristics of CNS in automatic milking systems (AMS) herds is sparse The role of CNS on the risk to acquire of IMI with S. aureus is debated.OBJECTIVES 1) To investigate the distribution of CNS species from aseptically collected quarter milk samples and teat skin in AMS herds, 2) To examine if the isolated CNS influence the expression of S. aureus virulence factors controlled by the agr quorum sensing system.This research study was supported by the Danish Milk Levy Foundation View publication stats

Coagulase negative staphylococci distribution in dairy herds with automatic milking system and their crosstalk with Staphylococcus aureus from IMI and teat apex

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Coagulase negative staphylococci distribution in dairy herds with automatic milking system and their crosstalk with Staphylococcus aureus from IMI and teat apex

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