<b>Effects of Wnt, BMP and Notch signaling on intestinal differentiation of <i>ex vivo</i> guts</b>. Small intestine from E13.5 embryos were isolated and cultured in medium containing 100 nM LDN-193189 (BMP signaling inhibitor; a), 10 μM DAPT (Notch signaling inhibitor; b), or 100 ng/ml Wnt 3a plus 500 ng/ml R-spondin1 (Wnt signaling activator; c). Relative mRNA expressions were tested using RT-qPCR. Data are represented as mean ± SEM. N=4. *: P < 0.05 versus CON. **: P < 0.01 versus CON
