MOESM4 of Paeoniflorin exerts neuroprotective effects by modulating the M1/M2 subset polarization of microglia/macrophages in the hippocampal CA1 region of vascular dementia rats via cannabinoid receptor 2
Additional file 4: Figure S3. Effects of paeoniflorin on the activation of microglial cells in hippocampi of rats after cerebral ischemia. One week after four-vessel occlusion (4-VO) surgery, rats were intraperitoneally administered saline (4-VO), paeoniflorin (4-VO+PF; 40 mg/kg/d), paeoniflorin+AM630 (4-VO+PF+AM630; 40 + 3 mg/kg/d), AM630 (4-VO+AM630; 3 mg/kg/d) or HU308 (4-VO+HU308; 3 mg/kg/d) for consecutive 28 days. After treatment, hippocampus tissue sections were co-stained for Iba1 (an activated microglia marker; green) or DAPI (blue). The images were observed and captured by a confocal laser scanning microscope. Scale bar: 50 Îźm
