Contient une table des matièresAvec mode text

Andrea Kuehn (621792)

Anne Almeida (4987679)

Arthur Castro (4987667)

Erick Figueiredo (4987676)

Ingrid Felger (92160)

Ivo Mueller (46609)

Larissa Brasil (4987670)

Marcelo Brito (3909610)

Marcus Lacerda (742724)

Quique Bassat (89288)

Sheila Vitor-Silva (326120)

Vanderson Sampaio (4722609)

Wanderli Tadei (4987673)

Wuelton Monteiro (752733)

Parasites & Vectors

BACKGROUND: Population-based studies conducted in Latin America
                have shown a high proportion of asymptomatic and submicroscopic
                malarial infections. Considering efforts aiming at regional
                elimination, it is important to investigate the role of this
                asymptomatic reservoir in malaria transmission in peri-urban
                areas. This study aimed to estimate the prevalence of Plasmodium
                spp. and gametocyte burden on symptomatic and asymptomatic
                infections in the Brazilian Amazon. RESULTS: Two cross-sectional
                household surveys (CS) were conducted including all inhabitants
                in a peri-urban area of Manaus, western Amazonas State, Brazil.
                Malaria parasites were detected by light microscopy (LM) and
                qPCR. Sexual stages of Plasmodium spp. were detected by LM and
                RT-qPCR. A total of 4083 participants were enrolled during the
                two surveys. In CS1, the prevalence of Plasmodium vivax
                infections was 4.3% (86/2010) by qPCR and 1.6% (32/2010) by LM.
                Fifty percent (43/86) of P. vivax infected individuals (qPCR)
                carried P. vivax gametocytes. In CS2, 3.4% (70/2073) of
                participants had qPCR-detectable P. vivax infections, of which
                42.9% (30/70) of infections were gametocyte positive. The P.
                vivax parasite density was associated with gametocyte carriage
                (P < 0.001). Sixty-seven percent of P. vivax infected
                individuals and 53.4% of P. vivax gametocyte carriers were
                asymptomatic. CONCLUSIONS: This study confirms a substantial
                proportion of asymptomatic and submicroscopic P. vivax
                infections in the study area. Most asymptomatic individuals
                carried gametocytes and presented low asexual parasitemia. This
                reservoir actively contributes to malaria transmission in the
                Brazilian Amazon, underscoring a need to implement more
                efficient control and elimination strategies

Almeida, Anne Cristine Gomes

Kuehn, Andrea

Castro, Arthur J. M.

Vitor Silva, Sheila

Figueiredo, Erick F. G.

Brasil, Larissa Wanderley

Brito, Marcelo A. M.

Sampaio, Vanderson Souza

Bassat Orellana, Quique

Felger, Ingrid

Tadei, Wanderli P.

Monteiro, Wuelton Marcelo

Mueller, Ivo

Lacerda, Marcus Vinícius Guimarães

Diposit Digital de la Universitat de Barcelona

High proportions of asymptomatic and submicroscopic Plasmodium
                vivax infections in a peri-urban area of low transmission in the
                Brazilian Amazon

Abstract Background Population-based studies conducted in Latin America have shown a high proportion of asymptomatic and submicroscopic malarial infections. Considering efforts aiming at regional elimination, it is important to investigate the role of this asymptomatic reservoir in malaria transmission in peri-urban areas. This study aimed to estimate the prevalence of Plasmodium spp. and gametocyte burden on symptomatic and asymptomatic infections in the Brazilian Amazon. Results Two cross-sectional household surveys (CS) were conducted including all inhabitants in a peri-urban area of Manaus, western Amazonas State, Brazil. Malaria parasites were detected by light microscopy (LM) and qPCR. Sexual stages of Plasmodium spp. were detected by LM and RT-qPCR. A total of 4083 participants were enrolled during the two surveys. In CS1, the prevalence of Plasmodium vivax infections was 4.3% (86/2010) by qPCR and 1.6% (32/2010) by LM. Fifty percent (43/86) of P. vivax infected individuals (qPCR) carried P. vivax gametocytes. In CS2, 3.4% (70/2073) of participants had qPCR-detectable P. vivax infections, of which 42.9% (30/70) of infections were gametocyte positive. The P. vivax parasite density was associated with gametocyte carriage (P < 0.001). Sixty-seven percent of P. vivax infected individuals and 53.4% of P. vivax gametocyte carriers were asymptomatic. Conclusions This study confirms a substantial proportion of asymptomatic and submicroscopic P. vivax infections in the study area. Most asymptomatic individuals carried gametocytes and presented low asexual parasitemia. This reservoir actively contributes to malaria transmission in the Brazilian Amazon, underscoring a need to implement more efficient control and elimination strategies

Anne C. G. Almeida

Andrea Kuehn

Arthur J. M. Castro

Sheila Vitor-Silva

Erick F. G. Figueiredo

Larissa W. Brasil

Marcelo A. M. Brito

Vanderson S. Sampaio

Quique Bassat

Ingrid Felger

Wanderli P. Tadei

Wuelton M. Monteiro

Ivo Mueller

Marcus V. G. Lacerda

Directory of Open Access Journals

High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon

Table S3. Conditions for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-qPCR assays. (DOCX 14 kb

FigShare

Additional file 3: of High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon

Monicault, Jacques de. Auteur du texte

Gallica, Bibliotheque Numerique

Le port de Beyrouth et l'économie des pays du Levant sous le mandat français : thèse pour le doctorat présentée et soutenue le 25 mai 1936... / par Jacques de Monicault... ; Université de Paris, Faculté de droit

Crossref

Background: Population-based studies conducted in Latin America have shown a high proportion of asymptomatic and submicroscopic malarial infections. Considering efforts aiming at regional elimination, it is important to investigate the role of this asymptomatic reservoir in malaria transmission in peri-urban areas. This study aimed to estimate the prevalence of Plasmodium spp. and gametocyte burden on symptomatic and asymptomatic infections in the Brazilian Amazon. Results: Two cross-sectional household surveys (CS) were conducted including all inhabitants in a peri-urban area of Manaus, western Amazonas State, Brazil. Malaria parasites were detected by light microscopy (LM) and qPCR. Sexual stages of Plasmodium spp. were detected by LM and RT-qPCR. A total of 4083 participants were enrolled during the two surveys. In CS1, the prevalence of Plasmodium vivax infections was 4.3% (86/2010) by qPCR and 1.6% (32/2010) by LM. Fifty percent (43/86) of P. vivax infected individuals (qPCR) carried P. vivax gametocytes. In CS2, 3.4% (70/2073) of participants had qPCR-detectable P. vivax infections, of which 42.9% (30/70) of infections were gametocyte positive. The P. vivax parasite density was associated with gametocyte carriage (P < 0.001). Sixty-seven percent of P. vivax infected individuals and 53.4% of P. vivax gametocyte carriers were asymptomatic. Conclusions: This study confirms a substantial proportion of asymptomatic and submicroscopic P. vivax infections in the study area. Most asymptomatic individuals carried gametocytes and presented low asexual parasitemia. This reservoir actively contributes to malaria transmission in the Brazilian Amazon, underscoring a need to implement more efficient control and elimination strategies. © 2018 The Author(s)

Castro, Arthur J.M.

Vítor-Silva, Sheila

Figueiredo, Erick F.G.

Brito, Marcelo Augusto Mota

S. Sampaio, Vanderson

Bassat, Quique

Tadei, Wanderli Pedro

Lacerda, Marcus V. G.

Repositório do INPA

Table S2. Reaction mixes for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-qPCR. The table describes the reagents, concentrations and template volume used in the qPCR and RT-qPCR assays. (DOCX 13 kb

Additional file 2: of High proportions of asymptomatic and submicroscopic Plasmodium vivax infections in a peri-urban area of low transmission in the Brazilian Amazon

Population-based studies conducted in Latin America have shown a high proportion of asymptomatic and submicroscopic malarial infections. Considering efforts aiming at regional elimination, it is important to investigate the role of this asymptomatic reservoir in malaria transmission in peri-urban areas. This study aimed to estimate the prevalence of Plasmodium spp. and gametocyte burden on symptomatic and asymptomatic infections in the Brazilian Amazon.; Two cross-sectional household surveys (CS) were conducted including all inhabitants in a peri-urban area of Manaus, western Amazonas State, Brazil. Malaria parasites were detected by light microscopy (LM) and qPCR. Sexual stages of Plasmodium spp. were detected by LM and RT-qPCR. A total of 4083 participants were enrolled during the two surveys. In CS1, the prevalence of Plasmodium vivax infections was 4.3% (86/2010) by qPCR and 1.6% (32/2010) by LM. Fifty percent (43/86) of P. vivax infected individuals (qPCR) carried P. vivax gametocytes. In CS2, 3.4% (70/2073) of participants had qPCR-detectable P. vivax infections, of which 42.9% (30/70) of infections were gametocyte positive. The P. vivax parasite density was associated with gametocyte carriage (P &lt; 0.001). Sixty-seven percent of P. vivax infected individuals and 53.4% of P. vivax gametocyte carriers were asymptomatic.; This study confirms a substantial proportion of asymptomatic and submicroscopic P. vivax infections in the study area. Most asymptomatic individuals carried gametocytes and presented low asexual parasitemia. This reservoir actively contributes to malaria transmission in the Brazilian Amazon, underscoring a need to implement more efficient control and elimination strategies

Le port de Beyrouth et l'économie des pays du Levant sous le mandat français : thèse pour le doctorat présentée et soutenue le 25 mai 1936... / par Jacques de Monicault... ; Université de Paris, Faculté de droit

Abstract

Similar works

Full text

Available Versions

Diposit Digital de la Universitat de Barcelona

Directory of Open Access Journals

FigShare

Gallica, Bibliotheque Numerique

Crossref

Repositório do INPA

FigShare

edoc