<p>Serial sections obtained from two paediatric control muscles (control 1 and 2, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0194540#pone.0194540.t001" target="_blank">Table 1</a>) were used in single or double labelling. (Single labelling: anti dystrophin ab15277, or anti dystrophin MANDYS106; double labelling: anti dystrophin ab15277 combined with anti spectrin, or anti dystrophin MANDYS106 combined with anti laminin). For each staining, two sections were stained simultaneously and four images were acquired per section using a fluorescent microscope and analysed by Metamorph software following the Arechavala et al., 2010 method. Sarcolemma intensity values were plotted on the y axis (Mean±SEM). Dystrophin intensity values plotted as dots are the difference between dystrophin intensity at the sarcolemma and dystrophin intensity within the cytoplasm (considered to be background staining signal). Analyses were performed using anti-dystrophin ab15277 (a), anti-dystrophin MANDYS106 (b), anti-spectrin (c) and anti-laminin (d).</p
