Additional file 1: of Hybridization capture reveals microbial diversity missed using current profiling methods

Abstract

Figure S1. Schematic representation of the hybridization capture method. Figure S2. Mock community profiles at different taxonomic levels for 16S rRNA gene amplicon sequencing, hybridization capture, and shotgun sequencing. Figure S3. Soil prokaryote composition profiles at different taxonomic levels for 16S rRNA gene amplicon sequencing, hybridization capture, and shotgun sequencing. Figure S4. Phylogenetic position of an unassigned sequence to a new phylum. Figure S5. Phylogenetic position of an unassigned sequence to a new class belonging to the Gemmatimonadetes phylum. Figure S6. Phylogenetic position of an unassigned sequence to a new class belonging to the Chloroflexi phylum. Figure S7. Phylogenetic position of new unassigned sequences to the Saccharibacteria phylum. Table S1. Microbial mock community used for hybridization capture validation and 16S rDNA relative abundances observed using the three methods (amplicons, capture, and shotgun sequencing). Table S2. Set of probes targeting the 16S rRNA gene used for hybridization capture. (DOCX 4852 kb