<p>Competitive RNA-EMSA was performed with recombinant PABP and <b>(A)</b> radiolabeled Ins2L-5’UTR RNA as probe in presence of increasing fold excess of unlabeled L or S RNA and <b>(B</b>) radiolabeled L2 (position 18–39) RNA as probe in presence of molar excess of unlabeled RNA (L, S, L1, L2, L3, L4). The shifted band was quantified and analyzed densitometrically and represented as the bar graph (lower panel). The values are an average of three independent experiments with error bars representing the mean ± SEM, and the significance indicated by p values. (<b>C</b>) m-fold predicted secondary structure of Ins2 splice variants (L/S). The boxed area shows the different folding of L2 region in both the splice variants.</p
