<p>MCE was induced in 3T3-L1 pre-adipocytes with differentiation medium, as described under “Materials and Methods”. Cells were then harvested at 12, 14, and 16 h after the initiation of differentiation in absence (Ctrl) or presence of <i>CA</i>de (100 μg/ml) and stained with PI solution for flow cytometer cell cycle analysis. <b>A</b>) Histograms of cell cycle distribution in G<sub>0</sub>/G<sub>1</sub>, S or G<sub>2</sub>/M phases. <b>B</b>) Quantitative analysis of cell cycle distribution. The results are means ± SD of three independent experiments. Statistical analysis was performed using Student’s t-test *<i>p</i><0.05, and ***<i>p</i><0.001, <i>CA</i>de S Phase <i>vs</i>. Ctrl S Phase; <sup>#</sup><i>p</i><0.05, <i>CA</i>de G<sub>2</sub>/M Phase <i>vs</i>. Ctrl G<sub>2</sub>/M Phase.</p
