<p>3T3-L1 pre-adipocytes were differentiated into mature adipocytes for 8 days, as described under “Materials and Methods”, in absence (Ctrl) or presence of <i>CA</i>de (100 μg/ml) from D0 to D2 (D0-D2), from D2 to D8 (D2-D8) and from D0 to D8 (D0-D8). <b>A</b>) Total TG deposition of mature adipocytes. Data are mean ± SD of determinations from three independent experiments. Statistical analysis was performed using one-way ANOVA. ***<i>p</i><0.001, <i>vs</i>. Ctrl. Microscopic images (10X magnification) (<b>B</b>), and lipid quantization (<b>C</b>) of mature adipocytes stained with Oil-Red O. The results are means ± SD of the Oil-red O absorbance values measured at 490 nm from three independent experiments and are expressed as fold changes over control. Statistical analysis was performed using one-way ANOVA. **<i>p</i><0.01 <i>vs</i>. Ctrl. qPCR was performed to detect the mRNA expression of (<b>D</b>) <i>Pparγ</i> at D4, and (<b>E</b>) <i>Glut4</i> and (<b>F</b>) <i>Fapb4</i> at D8 of the adipogenesis. Results are means ± SD of three independent experiments and are expressed as relative changes over control. Statistical analysis was performed using one-way ANOVA. *<i>p</i><0.05, **<i>p</i><0.01, and ***<i>p</i><0.001 <i>vs</i>. Ctrl at D2, D4 or D8. <b>G</b>) The uptake of 2-DG was then evaluated in mature adipocytes upon stimulation with insulin (100 nmol/l; Ins) for 30 min. The results are means ± SD of three independent experiments. Statistical analysis was performed using one-way ANOVA. ***<i>p</i><0.001 <i>vs</i>. Ctrl—Ins; <sup>###</sup><i>p</i><0.001, <i>CA</i>de D0-D8 + Ins <i>vs</i>. <i>CA</i>de D0-D8—Ins; <sup><i>¶¶¶</i></sup><i>p</i><0.001, <i>CA</i>de D0-D2 + Ins <i>vs</i>. <i>CA</i>de D0-D2—Ins; <sup>†††</sup><i>p</i><0.001, <i>CA</i>de D2-D8 + Ins <i>vs</i>. <i>CA</i>de D2-D8—Ins; <sup>§</sup><i>p</i><0.05 <i>vs</i>. Ctrl + Ins.</p
