<div>In Ayurveda, the leaf juice of Adhatoda vasica, a shrub native to Asia is incorporated in</div><div>many traditional herbal formulations. However, suitable solvent and a suitable extraction</div><div>method for phytochemical profiling are not well established, and there is no published mass</div><div>spectra structural interpretation of the identified compounds. This has caused a few</div><div>problems in herbal formulation research due to the bias derived from different extraction</div><div>methods. Therefore, this study used polar and non polar extraction for phytochemical</div><div>analysis on Adhatoda vasica, aiming to assess the potential impact of different solvents. This</div><div>study included extractive value, total phenol and alkaloid content of the leaves in different</div><div>preparations. Gas Chromatography coupled with Mass Spectrometry (GC-MS) was used to</div><div>study the phytochemical profile of different solvents. Significant differences were observed in</div><div>all the parameters such as extract yield, total phenol, total alkaloid and phytochemical</div><div>composition. The ethanol extract stood out most for effective extraction of phytochemicals,</div><div>especially for the alkaloids. The results highlight the necessity for comparative analyses of</div><div>chemical composition in different solvent extractions and careful choice and validation of</div><div>analytical methodology in herbal formulation research.</div
