<div>Mammalian models are frequently used for drug research and delivery systems. However, valid mammalian</div><div>models are expensive, time consuming and not easy to set up and evaluate. Furthermore, they are often linked</div><div>with ethical and legal aspects. Many micro, macro nutrients and other photochemical from food materials</div><div>are found to possess many desirable health benefits. A large number of studies involving animals have</div><div>been carried out to explore these beneficial properties. As an alternative, chick embryo developed through</div><div>incubation of fertile chicken eggs has been used as a novel system to develop suitable models for biochemical</div><div>and neutraceutical research. The in ovo technique is accepted as an alternative to traditional mammalian</div><div>models and this can be suitably used in our routine biochemical tests, wherever the animal trials are not</div><div>feasible. In view of the above, the development and evaluation of certain bioactive components using in ovo</div><div>technique as a diabetic model was carried out in our laboratory. Bixin decreased significantly the plasma</div><div>glucose, triglycerides, cholesterol, urea, creatinine and blood urea nitrogen, while liver glycogen levels in</div><div>treated diabetic chick embryos increased when compared to control diabetic chick embryos.</div
