Claeys, Erik

De Neve, Nympha

Fievez, Veerle

Gadeyne, Frederik

Van der Meeren, Paul

Vlaeminck, Bruno

Ghent University Academic Bibliography

New approach for polyphenol oxidase-mediated rumen bypass polyunsaturated fatty acids Nympha De Neve,a Frederik Gadeyne,a Bruno Vlaeminck,a Erik Claeys,a Paul Van der Meeren,b Veerle Fieveza aLaboratory for Animal Nutrition and Animal Product Quality, Ghent University, Coupure Links 653 Blok F, 9000 Ghent, Belgium bParticle and Interfacial Technology Group, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653 Blok B, 9000 Ghent, Belgium Previously, in vitro ruminal protection (>80 %) of PUFA was obtained by i) emulsifying a PUFA-rich oil with protein extract from potato tuber peel, containing polyphenol oxidase (PPO), and ii) inducing enzymatic cross-linking of the interfacial protein by addition of the PPO substrate 4-methylcatechol (4MC). In the current study, it was hypothesized better protein emulsifiers can adopt the emulsifying function, and PPO extract can be added after emulsification together with its substrate.  Linseed oil (10 wt%) emulsions were prepared using a protein emulsifier (1 wt%) (soy glycinin, gelatin, whey protein isolate, bovine serum albumin or sodium caseinate) and were diluted five times with PPO extract that was obtained from potato tuber peel by acetone precipitation. A linseed oil (2 wt%) emulsion with PPO extract only was used as the positive control. Protein adsorption behavior was analyzed by non-reducing SDS-PAGE and zymography. Emulsions were incubated for 24 h with 0 (negative control) or 40 mM 4MC. Droplet size distributions were characterized by laser diffraction and light microscopy. Rumen metabolism was simulated by 24 h in vitro incubation with rumen fluid. All emulsions were prepared and characterized in triplicate. Results were analyzed by the MIXED procedure of SAS.  Several treatments, including the control, showed an increase in droplet size after cross-linking in presence of 40 mM 4MC, due to droplet aggregation (p < 0.001). In all negative control emulsions (0 mM 4MC) PUFA were extensively biohydrogenated (94%-96%). In presence of 40 mM 4MC, compared to the positive control (81%) protection efficiencies were equal or higher for all treatments (p = 0.017, 86%-93%). Partitioning of PPO isoforms was similar for all treatments, showing low molecular weight PPO isoforms in the adsorbed protein fraction. Protein emulsifier can successfully adopt the emulsion function of PPO for ruminal protection, but even when PPO was added after emulsification, some PPO was present at the droplet interface. 

Protecting polyunsaturated fatty acids against biohydrogenation by polyphenol-oxidase-mediated cross-linking of interfacial protein : interfacial characterization

https://biblio.ugent.be/publication/8550826/file/8550830

Protecting polyunsaturated fatty acids against biohydrogenation by polyphenol-oxidase-mediated cross-linking of interfacial protein : interfacial characterization

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Ghent University Academic Bibliography