GeneChip results were confirmed for some genes using Northern blotting and RT-PCR

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Genomic analysis of the secretion stress response in the enzyme-producing cell factory "</p><p>BMC Genomics 2007;8():158-158.</p><p>Published online 11 Jun 2007</p><p>PMCID:PMC1894978.</p><p></p> Examples are provided for both Northern blots (A) and RT-PCR (B). Note that the RT-PCR for the mRNA was designed to indicate enhanced splicing of the mRNA intron under stress conditions (DTT, tunicamycin and production of t-PA). This is shown as a relative increase in the amount of the processed (lower band) form of the mRNA compared to the unprocessed higher band (). Probing or PCR for an actin gene was used as a non-stress-responsive control transcript

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