(A) Effect of protein synthesis inhibition on the downregulation of Nrl and Crx by TSA

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Requirement of histone deacetylase activity for the expression of critical photoreceptor genes"</p><p>http://www.biomedcentral.com/1471-213X/7/78</p><p>BMC Developmental Biology 2007;7():78-78.</p><p>Published online 29 Jun 2007</p><p>PMCID:PMC1914050.</p><p></p> P2 retinal explant cultures were treated with TSA, TSA plus cycloheximide, or DMSO for 3 hours, followed by Northern blot analysis. (B) Effect of HDAC inhibition on histone acetylation by western blot analysis. (C) Effect of HDAC inhibition on the acetylation of non-histone proteins by western blot analysis. The blot was sequentially probed with a pan acetyl-lysine antibodyn (top blot), an acetyl-tubulin antibody (middle blot), and an α-tubulin antibody (bottom blot)

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