60 μg protein were separated by precast NuPAGE Novex 4–12% Bis-Tris gels and transferred onto nitrocellulose membranes using the XCell II blot system

Abstract

The activation of glial cells was assessed by staining for the macrophage activation with antibodies against the F4/80 antigen. Immunoblot analysis revealed a decline of F4/80 in all CNI-1493 animals. Equal protein loading was assessed by reprobing the membrane with monoclonal antibodies against GAPDH.<p><b>Copyright information:</b></p><p>Taken from "CNI-1493 inhibits Aβ production, plaque formation, and cognitive deterioration in an animal model of Alzheimer's disease"</p><p></p><p>The Journal of Experimental Medicine 2008;205(7):1593-1599.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2442637.</p><p></p