Western blot analysis showing estrogen receptor (ER)-α expression in mammary gland extracts from parous rats treated with insulin-like growth factor (IGF)-I (P-IGF-I), untreated parous rats (P-Un), and age-matched virgin rats (AMV)

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Insulin-like growth factor (IGF)-I obliterates the pregnancy-associated protection against mammary carcinogenesis in rats: evidence that IGF-I enhances cancer progression through estrogen receptor-α activation via the mitogen-activated protein kinase pathway"</p><p>Breast Cancer Research 2004;6(4):R423-R436.</p><p>Published online 4 Jun 2004</p><p>PMCID:PMC468665.</p><p>Copyright © 2004 Thordardson et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.</p> The IGF-I treatment (0.66 mg/kg body weight/day) was continued for 7 days before samples were collected. The protein samples (100 μg/lane) were electrophorized on 7.5% SDS-PAGE, transferred to PVDF membrane, and probed with antibody specific to ER-α(Ab-15; NeoMarkers Inc., Fremont, CA, USA). Protein bands were detected using enhanced chemiluminescence reagents (upper image) and quantified using the ImageJ (version 1.24o; National Institutes of Health, Bethesda, MD, USA) image analysis program (bar chart). Values are expressed as mean ± standard error. *< 0.05 versus P-IGF-I and P-Un