Expression analysis of , , and , respectively, in overexpressing lines from 28-d-old soil-grown plants with northern blot hybridization and q-RT-PCR, respectively
(A) Wild-type and empty-vector plants served as controls. RNA from 10 plants per treatment was extracted and 10 μg total RNA was subjected to northern blot analysis. Gene-specific probes were radioactively labelled and hybridized as described in Materials and methods. Equal loading in each track is demonstrated by comparing the amount of the 28S rRNA. Transcript sizes are given in brackets. (B) Quantification of gene expression in transgenic plants overexpressing , , and , respectively. Gene expression was assessed by q-RT-PCR as described in Materials and methods. The data represent the average relative to the gene expression in control plants.<p><b>Copyright information:</b></p><p>Taken from "Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism"</p><p></p><p>Journal of Experimental Botany 2008;59(10):2831-2846.</p><p>Published online Jan 2008</p><p>PMCID:PMC2486478.</p><p></p
