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Pulsed-field Gel Electrophoresis

Abstract

Purpose: To describe the One-Day (24-26 hour) Standardized Laboratory Protocol for Molecular Subtyping of Vibrio cholera and Vibrio parahaemolyticus by Pulsed-field Gel Electrophoresis (PFGE).Scope: To provide the PulseNet participants with the same procedure for performing PFGE of Vibrio cholerae and Vibrio parahaemolyticus, thus ensuring inter-laboratory comparability of the generated results.PNL06 Last Updated April 2013vibrio_pfge_protocol-508c.pd

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