Zataria multiflora Boiss (Lamiaceae) commonly grows in Iran is a popular medicinal plant with various
pharmacological activities mentioned in traditional Iranian medicine and modern phytotherapy.
This study was designed to evaluate the chemical composition and scolicidal effects of Z. multiflora
essential oil on the protoscoleces of hydatid cysts on an in vitro model. The components of the Z.
multiflora essential oil were identified by GC/MS analysis. Protoscoleces were aseptically aspirated
from the livers of naturally infected sheep. Various concentrations of essential oil, thymol and
carvacrol were used for 5–30 minutes. Eosin exclusion test was used to determine the viability
of protoscoleces. The main components were thymol (41.8%), carvacrol (28.8%), and p-cymene
(8.4%). Findings showed that essential oil at the concentrations of 12.5 and 6.25 μL/mL killed 100%
protoscoleces after 5 and 20 minutes of exposure, respectively. In addition, thymol and carvacrol
at the concentrations of 100 μg/mL and 100 μL/mL killed 100% protoscoleces after 10 minutes
incubation, respectively. Obtained results in this investigation for the first time demonstrated that
Z. multiflora essential oil and its main components might be a natural source for the production of
new scolicidal agent
