PENGARUH PEMBERIAN ECHINACEA TERHADAP PERBEDAAN EKSPRESI GRANZYME DAN PERKEMBANGAN MASSA TUMOR PADA ADENOKARSINOMA MAMMA MENCIT C3H YANG MENGALAMI STRESS

Abstract

Echinacea contains some active compounds which could induce mononuclear cells activiy. Patients with breast carcinoma and stress, the immunity was depress. Echinacea will increase mononuclear cell granzyme expression. Granzyme is able to induce apoptosis that will influence tumour mass progression. Objectives : To prove the influence of Echinacea on mononuklear cell granzyme expression and tumour mass in C3H mice with breast carcinoma and stress. Methods: Pre and post test control group design experiment in C3H mice. Control group: standard C3H mice, group 1: C3H mice with stress aroused by applying electric shock, group 2: C3H mice with stress aroused by applying electric shock and treated with Echinacea. After tumor inoculation, the subjects are treated accordingly, and afterwards mononuclear cell granzyme expression and tumour mass progression were measured. One way Anova test and difference test were applied for each group by using Post Hoc Bonferoni Test. Pearson correlation used as statistical analysis of granzyme expression and tumour mass progression Results: There was a significant difference in granzyme expression (p<0,001) between control group and group-1 and between group-1 and group-2, but no significant difference (p=0,363) between control group and group-2. There was a significant diffrence Tumour mass (p<0,001) between control group and group-1 and between group-1and group-2, but no significant difference (p=0,453)between control group and group-2. The statistically significant negative correlation between granzyme and tumour mass progression (cc -0,925). Conclusion: There was a significant elevation of granzyme expression production and lower tumour mass progression in mice treated with Echinacea sp. Keywords: Echinacea sp,granzyme, tumor mass progressio