<i>Utx</i> mutant alleles.

Abstract

<p>(A) Schematics of mouse mutations in <i>Utx</i>. Included are annotations and locations of where the protein would be mutated. Two <i>Utx</i> mutant alleles included a gene trap in intron 3 (X<i>^UtxGT1</i>) and a gene trap/floxed exon 3 (X<i>^UtxGT2fl</i>). A UTX protein annotation is illustrated at the top to indicate to positions of <i>Utx</i> alleles. A germline Cre recombinase deleted exon 3 in the X<i>^UtxGT2fl</i> background to create X<i>^UtxGT2Δ</i>. Additionally, the gene trap of X<i>^UtxGT2fl</i> was excised with Flp recombinase to create a standard floxed exon 3 (X<i>^Utxfl</i>) and Cre recombination created X<i>^UtxΔ</i>. (B) Western blotting of E18.5 liver demonstrates a complete loss of UTX in X<i>^UtxGT1</i> Y<i>^Uty+</i> lysates. RbBP5 was used as a loading control. (C) Western blotting of E10.5 whole embryo demonstrates a complete loss of UTX in X<i>^UtxΔ</i> Y<i>^Uty+</i> and X<i>^UtxΔ</i> X<i>^UtxΔ</i> lysates. RbBP5 was used as a loading control. (D) Western blotting of E12.5 primary MEFs demonstrates a reduction of UTX in X<i>^UtxGT2fl</i> Y<i>^Uty+</i> and X<i>^UtxGT2fl</i> X<i>^UtxGT2fl</i> lysates. RbBP5 was used as a loading control.</p