Surface Charge Dependent
Nanoparticle Disruption and
Deposition of Lipid Bilayer Assemblies
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Abstract
Electrostatic interaction plays a leading role in nanoparticle
interactions with membrane architectures and can lead to effects such
as nanoparticle binding and membrane disruption. In this work, the
effects of nanoparticles (NPs) interacting with mixed lipid systems
were investigated, indicating an ability to tune both NP binding to
membranes and membrane disruption. Lipid membrane assemblies (LBAs)
were created using a combination of charged, neutral, and gel-phase
lipids. Depending on the lipid composition, nanostructured networks
could be observed using in situ atomic force microscopy representing
an asymmetrical distribution of lipids that rendered varying effects
on NP interaction and membrane disruption that were domain-specific.
LBA charge could be localized to fluidic domains that were selectively
disrupted when interacting with negatively charged Au nanoparticles
or quantum dots. Disruption was observed to be related to the charge
density of the membrane, with a maximum amount of disruption occurring
at ∼40% positively charged lipid membrane concentration. Conversely,
particle deposition was determined to begin at charged lipid concentrations
greater than 40% and increased with charge density. The results demonstrate
that the modulation of NP and membrane charge distribution can play
a pivitol role in determining NP-induced membrane disruption and NP
surface assembly