Overlay of three mass spectra showing the ΔF508-specific mass signal changes generated in the T-specific cleavage reaction of the reverse strands

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Multiplexed discovery of sequence polymorphisms using base-specific cleavage and MALDI-TOF MS"</p><p>Nucleic Acids Research 2005;33(4):e38-e38.</p><p>Published online 24 Feb 2005</p><p>PMCID:PMC549577.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> The mass signal pattern is derived from a triplexed reaction encompassing exon 10, 21 and 24 of the CFTR gene. Three different individuals were analyzed, the upper mass spectrum representing a homozygous wild-type for ΔF508, a carrier of the ΔF508 mutation (middle) and an individual homoyzgous mutant ΔF508 (lower mass spectrum). Mass signals specific for the mutation are indicated with a line